Baker H, DeAngelis B, Frank O, Khalil M, Hutner S H, Baker E R
Department of Preventive Medicine, New Jersey Medical School, Newark 07107, USA.
Experientia. 1996 Jun 15;52(6):597-9. doi: 10.1007/BF01969736.
Using a prokaryote (Escherichia coli) and a metazoa-resembling eukaryote (Ochromonas danica), we surveyed antioxidants which might overcome redox stress imposed by menadione sodium bisulphite (MD) and buthionine sulphoximine (BSO). BSO oxidant stress was evident only in O. danica; MD oxidant stress was evident in both organisms. Glutathione, its precursors, e.g. cysteine, homocysteine, and 2-oxo-4-thiazolidine carboxylic acid, and red blood cells, emerged as prime antioxidants for relieving BSO and MD oxidant stress. BSO and MD oxidant activity and antioxidant-annulling effect in O. danica were judged comparable to those found in animal cells whereas the results E. coli were not entirely equivalent. The O. danica system emerged as a practical, rapid, and useful system for pinpointing oxidant stressors and antioxidants, and shows promise for studies with mammalian systems.
我们使用一种原核生物（大肠杆菌）和一种类似后生动物的真核生物（丹麦赭球藻），调查了可能克服由亚硫酸氢钠甲萘醌（MD）和丁硫氨酸亚砜胺（BSO）施加的氧化还原应激的抗氧化剂。BSO氧化应激仅在丹麦赭球藻中明显；MD氧化应激在两种生物体中均明显。谷胱甘肽、其前体（如半胱氨酸、同型半胱氨酸和2-氧代-4-噻唑烷羧酸）以及红细胞，成为缓解BSO和MD氧化应激的主要抗氧化剂。丹麦赭球藻中BSO和MD的氧化活性以及抗氧化剂消除作用被判定与动物细胞中的相当，而大肠杆菌的结果并不完全相同。丹麦赭球藻系统成为一种实用、快速且有用的系统，用于确定氧化应激源和抗氧化剂，并显示出在哺乳动物系统研究中的前景。
