Zhao X, Lein E S, He A, Smith S C, Aston C, Gage F H
Laboratory of Genetics, Vision Center Laboratory, the Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
J Comp Neurol. 2001 Dec 17;441(3):187-96. doi: 10.1002/cne.1406.
The hippocampus consists of distinct anatomic regions that have been demonstrated to have different biological functions. To explore the molecular differences between hippocampal subregions, we performed transcriptional profiling analysis by using DNA microarray technology. The cRNA derived from the CA1, CA3, and dentate gyrus regions of the hippocampus and from spinal cord was hybridized to Affymetrix high-density oligo arrays. This systematic approach revealed sets of genes that were expressed specifically in subregions of the hippocampus corresponding to predefined cytoarchitectural boundaries, which could be confirmed by in situ hybridization and Real Time quantitative polymerase chain reaction. The relative enrichment and absence of genes in the hippocampal subregions support the conclusion that there is a molecular basis for the previously defined anatomic subregions of the hippocampus and also reveal genes that could be important in defining the unique functions of the hippocampal subfields.
海马体由不同的解剖区域组成，这些区域已被证明具有不同的生物学功能。为了探究海马体亚区域之间的分子差异，我们使用DNA微阵列技术进行了转录谱分析。从海马体的CA1、CA3和齿状回区域以及脊髓获得的cRNA与Affymetrix高密度寡核苷酸阵列进行杂交。这种系统方法揭示了在与预先定义的细胞结构边界相对应的海马体亚区域中特异性表达的基因集，这可以通过原位杂交和实时定量聚合酶链反应得到证实。海马体亚区域中基因的相对富集和缺失支持了这样的结论，即海马体先前定义的解剖亚区域存在分子基础，并且还揭示了在定义海马体亚区独特功能方面可能重要的基因。
