Levine M Z, Harrison P J, Walthall W W, Tai P C, Derby C D
Department of Biology and Center for Behavioral Neuroscience, Georgia State University, Atlanta, Georgia 30303, USA.
J Neurobiol. 2001 Dec;49(4):277-302. doi: 10.1002/neu.10010.
csp, a gene encoding a protein with high sequence identity to trypsinlike serine protease and CUB domains, was identified from a cDNA library from the olfactory organ (antennular lateral flagellum) of the spiny lobster Panulirus argus. The full-length cDNA sequence of csp is 1801 bp, encoding a protein of 50.25 kD, with three domains: signal peptide, trypsinlike serine protease, and CUB (named for a class of compounds including Complement subcomponents Clr/Cls, Uegf, and Bone morphogenic protein-1). RT-PCR, Northern blots, and immunoblots showed that csp is predominantly expressed in the lateral flagellum and eyestalk. Immunocytochemistry showed that Csp is present in olfactory (aesthetasc) sensilla around auxiliary cells (glia that surround the inner dendrites of olfactory receptor neurons, ORNs) and ORN outer dendrites. We propose that Csp is expressed and secreted by auxiliary cells, associates with ORN cell membranes or extracellular matrix via the CUB domain, and has trypsinlike activity. In the eyestalk, Csp is associated with cells surrounding axons between neuropils of the eyestalk ganglia. Possible functions in the olfactory organ and eyestalk are discussed. To our knowledge, this is the first report from any olfactory system of a gene encoding a protein with serine protease and CUB domains.
从多棘龙虾（Panulirus argus）嗅觉器官（触角侧鞭）的cDNA文库中鉴定出csp基因，该基因编码一种与胰蛋白酶样丝氨酸蛋白酶和CUB结构域具有高度序列同一性的蛋白质。csp的全长cDNA序列为1801 bp，编码一个50.25 kD的蛋白质，具有三个结构域：信号肽、胰蛋白酶样丝氨酸蛋白酶和CUB（以包括补体亚成分Clr/Cls、Uegf和骨形态发生蛋白-1在内的一类化合物命名）。逆转录聚合酶链反应（RT-PCR）、Northern印迹和免疫印迹显示，csp主要在侧鞭和眼柄中表达。免疫细胞化学显示，Csp存在于辅助细胞（围绕嗅觉受体神经元ORN内树突的神经胶质细胞）周围的嗅觉（嗅毛）感受器和ORN外树突中。我们推测，Csp由辅助细胞表达和分泌，通过CUB结构域与ORN细胞膜或细胞外基质结合，并具有胰蛋白酶样活性。在眼柄中，Csp与眼柄神经节神经毡之间轴突周围的细胞相关联。文中讨论了其在嗅觉器官和眼柄中的可能功能。据我们所知，这是来自任何嗅觉系统的关于编码具有丝氨酸蛋白酶和CUB结构域蛋白质的基因的首次报道。
