McClintock T S, Xu F, Quintero J, Gress A M, Landers T M
Department of Physiology, University of Kentucky, Lexington 40536-0084, U.S.A.
J Neurochem. 1997 Jun;68(6):2248-54. doi: 10.1046/j.1471-4159.1997.68062248.x.
We have isolated from an American lobster (Homarus americanus) olfactory organ cDNA library a clone, hG alpha(q), with >80% identity to mammalian and arthropod G alpha(q) sequences. In brain and olfactory organ, hG alpha(q) mRNA was expressed predominantly in neurons, including virtually all the neuronal cell body clusters of the brain. G alpha(q) protein was also expressed broadly, appearing on western blots as a single band of 46 kDa in brain, eyestalk, pereiopod, dactyl, tail muscle, olfactory organ, and aesthetasc hairs. These results suggest that hG alpha(q) plays a role in a wide variety of signal transduction events. Its presence in the olfactory aesthetasc hairs, which are almost pure preparations of the outer dendrites of the olfactory receptor neurons, the expression of a single hG alpha(q) mRNA species (6 kb) in the olfactory organ, and the localization of hG alpha(q) mRNA predominantly in the olfactory receptor neurons of the olfactory organ strongly suggest that one function of hG alpha(q) is to mediate olfactory transduction.
我们从美洲龙虾（美洲螯龙虾）的嗅觉器官cDNA文库中分离出一个克隆，即hGα(q)，它与哺乳动物和节肢动物的Gα(q)序列具有80%以上的同源性。在脑和嗅觉器官中，hGα(q) mRNA主要在神经元中表达，包括脑中几乎所有的神经元细胞体簇。Gα(q)蛋白也广泛表达，在蛋白质印迹中，在脑、眼柄、步足、指节、尾肌、嗅觉器官和嗅觉刚毛中呈现为一条46 kDa的单带。这些结果表明hGα(q)在多种信号转导事件中发挥作用。它存在于嗅觉刚毛中，而嗅觉刚毛几乎是嗅觉受体神经元外树突的纯制剂，在嗅觉器官中表达单一的hGα(q) mRNA种类（6 kb），并且hGα(q) mRNA主要定位于嗅觉器官的嗅觉受体神经元中，这强烈表明hGα(q)的一个功能是介导嗅觉转导。
