Leegsma-Vogt G, Venema K, Postema F, Korf J
Department of Psychiatry, Groningen University, Hanzeplein 1, PO Box 30.001, 9700 RB Groningen, The Netherlands.
J Neurosci Res. 2001 Dec 1;66(5):795-802. doi: 10.1002/jnr.10046.
Continuous monitoring of arterio-venous glucose and lactate differences may serve as a diagnostic tool to assess normal brain function and brain pathology. We describe a method and some results obtained with arterio-venous measurements of glucose and lactate in the blood of the halothane-anesthetized rat and after brain injury. The method is based on low flow rate ultrafiltration for continuous collection of blood filtrate combined with flow injection analysis and biosensors for the detection of glucose and lactate. We measured the glucose and lactate concentration every minute in the jugular vein and the aorta at control conditions and during and after inflation of an embolectomy-balloon for 2 min. Net cerebral lactate efflux and glucose uptake was seen under control conditions and at low blood lactate levels. During brain injury both lactate release and glucose uptake were reduced and there was a net lactate influx at high arterial lactate levels. These results indicate that the flux of lactate in and out of the brain is not only dependent on the lactate concentration in the brain, but on blood levels as well, possibly because of bi-directional flux through the monocarboxylate transporter type 1.
持续监测动静脉葡萄糖和乳酸差异可作为评估正常脑功能和脑病理学的诊断工具。我们描述了一种方法以及在氟烷麻醉大鼠和脑损伤后通过动静脉测量血液中葡萄糖和乳酸获得的一些结果。该方法基于低流速超滤连续收集血液滤液，并结合流动注射分析和生物传感器来检测葡萄糖和乳酸。我们在对照条件下以及在栓塞切除球囊充气2分钟期间和之后，每分钟测量颈静脉和主动脉中的葡萄糖和乳酸浓度。在对照条件下和低血乳酸水平时可见脑乳酸净流出和葡萄糖摄取。在脑损伤期间，乳酸释放和葡萄糖摄取均减少，并且在高动脉乳酸水平时有乳酸净流入。这些结果表明，乳酸进出大脑的通量不仅取决于大脑中的乳酸浓度，还取决于血液水平，这可能是由于通过单羧酸转运蛋白1的双向通量所致。
