Witten P E, Hansen A, Hall B K
Department of Biology, Dalhousie University, 1355 Oxford St., Halifax Nova Scotia, Canada B3H 4J1.
J Morphol. 2001 Dec;250(3):197-207. doi: 10.1002/jmor.1065.
To provide basic data about bone resorbing cells in the skeleton during the life cycle of Danio rerio, larvae, juveniles, and adults (divided into six age groups) were studied by histological procedures and by demonstration of the osteoclast marker enzyme tartrate-resistant acid phosphatase (TRAP). Special attention was paid to the lower jaw, which is a standard element for fish bone studies. The presence of osteoclasts at endosteal surfaces of growing bones of all animals older than 20 days reveals that resorption is an important part of zebrafish skeletal development. The first bone-resorbing cells to form are mononucleated. They appear in 20-day-old animals concurrently in the craniofacial skeleton and vertebral column. Mononucleated osteoclasts are predominant in juveniles. Regional differences characterize the appearance of osteoclasts; at thin skeletal elements (neural arches, nasal) mononucleated osteoclasts are predominant even in adults. Multinucleated bone-resorbing cells were first observed in 40-day-old animals and are the predominant osteoclast type of adults. Both mono- and multinucleated osteoclasts contribute to allometric bone growth but multinucleated osteoclasts are also involved in lacunar bone resorption and repeated bone remodeling. Resorption of the dentary follows the pattern described above (mononucleated osteoclasts precede multinucleated cells) and includes the partial removal of Meckel's cartilage. Bone marrow spaces created by resorption are usually filled with adipose tissue. In conclusion, bone resorption is primarily subjected to the demands of growth, the appearance of mono- and multinucleated osteoclasts is site- and age-related, and bone remodeling occurs. The results are discussed in relation to findings in other teleosts and in mammals.
为了提供关于斑马鱼生命周期中骨骼骨吸收细胞的基础数据，通过组织学方法以及破骨细胞标志物酶抗酒石酸酸性磷酸酶（TRAP）的检测，对斑马鱼的幼体、幼鱼和成鱼（分为六个年龄组）进行了研究。特别关注了下颌骨，它是鱼类骨骼研究的标准部位。在所有20日龄以上动物的生长骨骼内骨表面存在破骨细胞，这表明骨吸收是斑马鱼骨骼发育的重要组成部分。最早形成的骨吸收细胞是单核的。它们在20日龄动物的颅面骨骼和脊柱中同时出现。单核破骨细胞在幼鱼中占主导地位。破骨细胞的出现具有区域差异；在薄的骨骼部位（神经弓、鼻骨），即使在成鱼中单核破骨细胞也占主导地位。多核骨吸收细胞最早在40日龄动物中观察到，并且是成鱼中主要的破骨细胞类型。单核和多核破骨细胞都参与了异速生长的骨生长，但多核破骨细胞也参与了腔隙性骨吸收和反复的骨重塑。齿骨的吸收遵循上述模式（单核破骨细胞先于多核细胞），并且包括梅克尔软骨的部分移除。吸收产生的骨髓腔通常充满脂肪组织。总之，骨吸收主要受生长需求的影响，单核和多核破骨细胞的出现与部位和年龄相关，并且发生了骨重塑。将这些结果与其他硬骨鱼和哺乳动物的研究结果进行了讨论。
