Balázs M, Horváth G, Grama L, Balogh P
Department of Immunology and Biotechnology, University Medical School of Pécs, Pécs, Hungary.
Cell Immunol. 2001 Sep 15;212(2):126-37. doi: 10.1006/cimm.2001.1847.
In this paper we report the development of the sinus network of mouse spleen during the first postnatal month as studied with a set of new rat monoclonal antibodies (mAbs) against mouse splenic endothelial cell subpopulations. One of the new mAbs (IBL-7/1) also stained B-cell lineage cells in the spleen shortly after the birth as confirmed by three-color flow cytometry. This B-cell staining in the primordial follicles vanished by the fourth postnatal week, so that the expression of IBL-7/1 antigen was restricted to the marginal sinus endothelium and some red pulp sinuses and a minor B-cell subset in the spleen, presumably distinct from the follicular B-cell compartment. The other mAb (IBL-9/2) selectively labeled the sinusoids of the deeper part of the red pulp, without any reactivity against hemopoietic cells. The IBL-9/2-reactive cells in newborns appeared as isolated elements throughout spleen, and during the segregation of white and red pulps they formed an extensive network in the red pulp outside the marginal zone. Double-labeling immunofluorescence revealed that most of these sinusoids also stained weakly with IBL-7/1 mAb, whereas the strongly IBL-7/1-positive vessels of this region were IBL-9/2 negative. Neither of these mAbs reacted with the central artery. The comparative phenotypic analysis of the various vascular segments indicates that the splenic sinusoids of the marginal zone and red pulp, respectively, are lined with a heterogeneous array of endothelium. For the precise identification, isolation, and characterization of the possible homing function of these endothelium subsets these region-specific mAbs may be of potential value.
在本文中，我们报告了出生后第一个月小鼠脾脏窦状网络的发育情况，该研究采用了一组针对小鼠脾脏内皮细胞亚群的新型大鼠单克隆抗体（mAb）。其中一种新型单克隆抗体（IBL-7/1）在出生后不久也能对脾脏中的B细胞谱系细胞进行染色，这一点通过三色流式细胞术得到了证实。出生后第四周，原始卵泡中的这种B细胞染色消失，因此IBL-7/1抗原的表达仅限于边缘窦内皮、一些红髓窦以及脾脏中一个较小的B细胞亚群，推测该亚群与滤泡B细胞区室不同。另一种单克隆抗体（IBL-9/2）选择性地标记红髓深部的血窦，对造血细胞无任何反应性。新生小鼠中IBL-9/2反应性细胞在整个脾脏中表现为孤立的细胞成分，在白髓和红髓分离过程中，它们在边缘区外的红髓中形成了广泛的网络。双标免疫荧光显示，这些血窦中的大多数也与IBL-7/1单克隆抗体呈弱阳性反应，而该区域强IBL-7/1阳性的血管则为IBL-9/2阴性。这两种单克隆抗体均不与中央动脉发生反应。对不同血管段的比较表型分析表明，边缘区和红髓的脾血窦分别由异质性的内皮细胞排列组成。对于这些内皮细胞亚群可能的归巢功能的精确鉴定、分离和表征，这些区域特异性单克隆抗体可能具有潜在价值。
