Wells S M, Kantor A B, Stall A M
Department of Microbiology, Columbia University, College of Physicians and Surgeons, New York, NY 10032-3702.
J Immunol. 1994 Dec 15;153(12):5503-15.
CD43 (leukosialin) expression has previously been demonstrated on the surface of developing B cells in mouse bone marrow and on plasma cells induced in vitro, but not on peripheral B cells in spleen. Here we show that CD43, as recognized by mAb S7, is indeed expressed on a small population of splenic B cells. Flow cytometric phenotyping of normal mice and radiation chimeras reveals that CD43/S7 is expressed on virtually all (> 90 to 95%) splenic B-1 cells and the majority of peritoneal B-1 cells, but not on conventional B cells. The expression of CD43/S7, in conjunction with other cell surface markers, clearly distinguishes B-1 cells from follicular, marginal zone, and immature B cells in the unstimulated adult spleen and permits further phenotyping of these subsets. The phenotype of splenic and peritoneal B-1 cells in normal BALB/c and BAB/25 mice is essentially identical with the exception that all peritoneal B-1 cells express CD11b (Mac-1) and some lack CD43/S7 and heat stable Ag (as detected by the mAb 53-10) expression. Although splenic B-1, marginal zone, and immature B cells share many phenotypic characteristics, these studies show that, in addition to CD43, they differ with respect to the expression levels of a variety of Ags including heat stable Ag, B220, and the B cell activation Ag B7.
先前已证明CD43（白细胞唾液酸蛋白）在小鼠骨髓中发育中的B细胞表面以及体外诱导的浆细胞上表达，但在脾脏中的外周B细胞上不表达。在此我们表明，单克隆抗体S7所识别的CD43确实在一小部分脾脏B细胞上表达。对正常小鼠和辐射嵌合体进行流式细胞术表型分析发现，CD43/S7在几乎所有（>90%至95%）脾脏B-1细胞和大多数腹膜B-1细胞上表达，但在传统B细胞上不表达。CD43/S7与其他细胞表面标志物共同表达，可在未受刺激的成年脾脏中清楚地区分B-1细胞与滤泡、边缘区和未成熟B细胞，并允许对这些亚群进行进一步的表型分析。正常BALB/c和BAB/25小鼠脾脏和腹膜B-1细胞的表型基本相同，只是所有腹膜B-1细胞均表达CD11b（Mac-1），且一些细胞缺乏CD43/S7和热稳定抗原（通过单克隆抗体53-10检测）的表达。尽管脾脏B-1、边缘区和未成熟B细胞具有许多表型特征，但这些研究表明，除了CD43外，它们在包括热稳定抗原、B220和B细胞活化抗原B7在内的多种抗原的表达水平上也存在差异。
