酸枣仁皂苷A对青霉素钠诱导的大鼠海马CA1区体外活动亢进的抑制作用
Inhibitory effect of jujuboside A on penicillin sodium induced hyperactivity in rat hippocampal CA1 area in vitro.
作者信息
Shou C H, Wang J, Zheng X X, Guo D W
机构信息
Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China.
出版信息
Acta Pharmacol Sin. 2001 Nov;22(11):986-90.
AIM
To study the effect of jujuboside A (JuA), one constituent of Chinese herbal medicine Ziziphus jujuba Mill Var spinosa (Bunge) Hu,on the penicillin sodium induced hyperactivity in rat CA1 neurons in vitro.
METHODS
Hippocampal slices were obtained from the Sprague-Dawley rat brain and populational signals were measured from CA1 neurons of hippocampal slices using the extracellular recording technique.
RESULTS
Penicillin sodium of 500, 1000, and 2000 kU/L were found to excite hippocampal CA1 neurons in a concentration-dependent manner in vitro. This excitatory effect of penicillin sodium could be inhibited by phenobarbital sodium of 0.02 - 0.05 g/L and JuA of 0.05 - 0.10 g/L.
CONCLUSION
A high dose of JuA can inhibit the hyperactivity of hippocampal CA1 area induced by penicillin sodium. The inhibition of the amplitude of the first population spike (PS) and the latency of PS are more pronounced than the slope of the field excitatory post-synaptic potential.
目的
研究中药酸枣仁的成分之一酸枣仁皂苷A（JuA）对青霉素钠诱导的大鼠离体CA1神经元活动亢进的影响。
方法
从Sprague-Dawley大鼠脑获取海马脑片，采用细胞外记录技术测量海马脑片CA1神经元的群体信号。
结果
发现500、1000和2000 kU/L的青霉素钠在体外以浓度依赖性方式兴奋海马CA1神经元。0.02 - 0.05 g/L的苯巴比妥钠和0.05 - 0.10 g/L的JuA可抑制青霉素钠的这种兴奋作用。
结论
高剂量的JuA可抑制青霉素钠诱导的海马CA1区活动亢进。对第一个群体峰电位（PS）的幅度和PS的潜伏期的抑制比对场兴奋性突触后电位的斜率的抑制更明显。
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