Williams Kelly P
Department of Biology, Indiana University, 1001 E. Third Street, Bloomington, IN 47405, USA.
Nucleic Acids Res. 2002 Jan 1;30(1):179-82. doi: 10.1093/nar/30.1.179.
tmRNA (also known as 10Sa RNA or SsrA) plays a central role in an unusual mode of translation, whereby a stalled ribosome switches from a problematic mRNA to a short reading frame within tmRNA during translation of a single polypeptide chain. Research on the mechanism, structure and biology of tmRNA is served by the tmRNA Website, a collection of sequences for tmRNA and the encoded proteolysis-inducing peptide tags, alignments, careful documentation and other information; the URL is http://www.indiana.edu/~tmrna. Four pseudoknots are usually present in each tmRNA, so the database is rich with information on pseudoknot variability. Since last year it has doubled (227 tmRNA sequences as of September 2001), a sequence alignment for the tmRNA cofactor SmpB has been included, and genomic data for Clostridium botulinum has revealed a group I (subgroup IA3) intron interrupting the tmRNA T-loop.
转移信使核糖核酸(也称为10Sa RNA或SsrA)在一种不同寻常的翻译模式中起着核心作用,即停滞的核糖体在单个多肽链翻译过程中从有问题的信使核糖核酸切换到转移信使核糖核酸内的短阅读框。转移信使核糖核酸网站(http://www.indiana.edu/~tmrna)为转移信使核糖核酸的机制、结构和生物学研究提供服务,该网站收集了转移信使核糖核酸和编码的诱导蛋白水解肽标签的序列、比对、详细文档及其他信息。每个转移信使核糖核酸通常有四个假结,因此该数据库富含假结变异性的信息。自去年以来,其规模扩大了一倍(截至2001年9月有227个转移信使核糖核酸序列),已纳入转移信使核糖核酸辅因子SmpB的序列比对,并且肉毒梭菌的基因组数据显示有一个I组(IA3亚组)内含子中断了转移信使核糖核酸的T环。