Pesole Graziano, Liuni Sabino, Grillo Giorgio, Licciulli Flavio, Mignone Flavio, Gissi Carmela, Saccone Cecilia
Dipartimento di Fisiologia e Biochimica Generali, Università di Milano, via Celoria 26, 20133 Milano, Italy.
Nucleic Acids Res. 2002 Jan 1;30(1):335-40. doi: 10.1093/nar/30.1.335.
The 5'- and 3'-untranslated regions (5'- and 3'-UTRs) of eukaryotic mRNAs are known to play a crucial role in post-transcriptional regulation of gene expression modulating nucleo-cytoplasmic mRNA transport, translation efficiency, subcellular localization and stability. UTRdb is a specialized database of 5' and 3' untranslated sequences of eukaryotic mRNAs cleaned from redundancy. UTRdb entries are enriched with specialized information not present in the primary databases including the presence of nucleotide sequence patterns already demonstrated by experimental analysis to have some functional role. All these patterns have been collected in the UTRsite database so that it is possible to search any input sequence for the presence of annotated functional motifs. Furthermore, UTRdb entries have been annotated for the presence of repetitive elements. All Internet resources we implemented for retrieval and functional analysis of 5'- and 3'-UTRs of eukaryotic mRNAs are accessible at http://bighost.area.ba.cnr.it/BIG/UTRHome/.
真核生物mRNA的5'和3'非翻译区(5'-和3'-UTR)在基因表达的转录后调控中发挥着关键作用,可调节核质mRNA转运、翻译效率、亚细胞定位和稳定性。UTRdb是一个专门的数据库,收录了去除冗余后的真核生物mRNA的5'和3'非翻译序列。UTRdb条目富含初级数据库中不存在的专门信息,包括已通过实验分析证明具有某些功能作用的核苷酸序列模式。所有这些模式都已收集在UTRsite数据库中,因此可以搜索任何输入序列中是否存在注释的功能基序。此外,UTRdb条目还针对重复元件的存在进行了注释。我们为真核生物mRNA的5'-和3'-UTR的检索和功能分析所实现的所有互联网资源可在http://bighost.area.ba.cnr.it/BIG/UTRHome/上获取。