Hendrickx A, Bossuyt X
Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium.
Cytometry. 2001 Dec 15;46(6):336-9. doi: 10.1002/cyto.10032.
CD45 is a glycoprotein expressed on all lymphohematopoietic cells. Its expression increases during normal B-cell differentiation and remains stable on mature cells. Although it is widely known that CD45 antigen expression is decreased in B-acute lymphocytic leukemia (ALL), only scarce and contradictory information is available on CD45 expression on mature B-cell malignancies. In healthy adults (n = 15), CD45 expression on B lymphocytes was lower than that on T cells. In patients with chronic lymphocytic leukemia (CLL; n = 22), CD45 expression on malignant cells was lower than that on the whole lymphocyte population of healthy adults (n = 28) and on normal B lymphocytes (n = 15). In 6 of the 22 CLL patients, the malignant cell population could be separated from the normal lymphocyte population on the CD45-side scatter (SSC) plot. In 16 CLL patients, there was some degree of overlap between the malignant and normal cells with respect to CD45 expression. For these patients, there was an inverse correlation between CD45 expression on the whole lymphocyte population and the percentage of malignant cells in this population. In two patients with mantle cell lymphoma (MCL), CD45 expression on the malignant cells appeared lower than that on normal B cells and on the whole lymphocyte population. In six patients with hairy cell leukemia (HCL), CD45 expression on hairy cells was comparable to that on the whole lymphocyte population of healthy adults, but slightly higher than that of normal B cells. Evaluation of CD45 expression may help to characterize mature B-cell malignancies.
CD45是一种在所有淋巴细胞造血细胞上表达的糖蛋白。其表达在正常B细胞分化过程中增加，并在成熟细胞上保持稳定。尽管众所周知CD45抗原表达在B淋巴细胞急性白血病（ALL）中降低，但关于成熟B细胞恶性肿瘤中CD45表达的信息却稀少且相互矛盾。在健康成年人（n = 15）中，B淋巴细胞上的CD45表达低于T细胞。在慢性淋巴细胞白血病（CLL；n = 22）患者中，恶性细胞上的CD45表达低于健康成年人（n = 28）的全淋巴细胞群体以及正常B淋巴细胞（n = 15）。在22例CLL患者中的6例，恶性细胞群体在CD45-侧向散射（SSC）图上可与正常淋巴细胞群体分离。在16例CLL患者中，恶性细胞与正常细胞在CD45表达方面存在一定程度的重叠。对于这些患者，全淋巴细胞群体上的CD45表达与该群体中恶性细胞的百分比呈负相关。在两名套细胞淋巴瘤（MCL）患者中，恶性细胞上的CD45表达似乎低于正常B细胞和全淋巴细胞群体。在六名毛细胞白血病（HCL）患者中，毛细胞上的CD45表达与健康成年人的全淋巴细胞群体相当，但略高于正常B细胞。评估CD45表达可能有助于对成熟B细胞恶性肿瘤进行特征描述。
