Caldwell C W, Patterson W P
Department of Pathology, University of Missouri School of Medicine, Columbia 65212.
Am J Hematol. 1991 Feb;36(2):111-5. doi: 10.1002/ajh.2830360209.
The cell-surface antigen CD45 is a complex family of high-molecular-weight glycoproteins expressed on all lymphohematopoietic cells, but not in the same molecular isoform. This antigen complex is known to exhibit protein tyrosine phosphatase (PTPase) activity and appears to have a role in regulation of cell differentiation. In that CD45 expression parallels stages of differentiation in normal bone marrow B cells, it was of interest to evaluate this process in malignant B cells. Monoclonal antibodies (MoAbs) were used to investigate the quantitative expression of CD45 and CD45RA on the B cells of lymphoid leukemias. Employing standardized flow cytometric methods, it was found that the fluorescence intensity (FI) of immunostained malignant B cells, as a reflection of the antigen content, demonstrated correlations with the putative stage of cell differentiation for malignancies at the earlier stages, but at the later stages, a progressive loss of CD45 was observed. Since this antigen family has been found to display PTPase activity, further investigation of CD45 alterations in malignancies may provide insight into potential regulatory disturbances.
细胞表面抗原CD45是一个高分子量糖蛋白的复杂家族，在所有淋巴造血细胞上表达，但分子异构体不同。已知这种抗原复合物具有蛋白酪氨酸磷酸酶（PTPase）活性，并且似乎在细胞分化调节中起作用。鉴于CD45的表达与正常骨髓B细胞的分化阶段平行，因此评估恶性B细胞中的这一过程很有意义。使用单克隆抗体（MoAbs）来研究淋巴白血病B细胞上CD45和CD45RA的定量表达。采用标准化的流式细胞术方法，发现免疫染色的恶性B细胞的荧光强度（FI）作为抗原含量的反映，在早期阶段与恶性肿瘤细胞分化的假定阶段相关，但在后期阶段，观察到CD45逐渐丢失。由于已发现该抗原家族具有PTPase活性，因此进一步研究恶性肿瘤中CD45的改变可能有助于深入了解潜在的调节紊乱。
