Ranuncolo Stella Maris, Ladeda Virginia, Specterman Sergio, Varela Mirta, Lastiri José, Morandi Ana, Matos Elena, Bal de Kier Joffé Elisa, Puricelli Lydia, Pallotta María Guadalupe
Research Area of the Institute of Oncology Angel H. Roffo, Buenos Aires, Argentina.
J Surg Oncol. 2002 Jan;79(1):30-5; discussion 35-6. doi: 10.1002/jso.10045.
Glioma invasiveness involves the attachment of tumor cells to the brain extracellular matrix, rich in hyaluronic acid (HA). CD44, the principal receptor for HA is found as a standard molecule (CD44s) or as variants (CD44v). We undertook a retrospective study to evaluate the expression pattern of CD44s, the isoforms CD44v3, v4/5 and v6 and to correlate their expression with clinical-anatomopathological parameters and survival rate.
The expression of these molecules was evaluated immunohistochemically in 84 gliomas.
No expression of CD44v was detected in any tumors. CD44s staining of tumor cells was found in 70 of 84 (83.3%) of the gliomas. In 23 of 39 (59.0%) of the GBM more than the 70% of the cells were stained, while only 2 of 21 (9.5%) of LGA showed high expression. The association between CD44 and histological grade remained when the prognostic variables were considered in a multivariate analysis. Higher expression of CD44 was associated with worse overall survival rate; however, the Cox analysis indicated that survival was not associated with CD44.
Our results suggest that overexpression of CD44s could be relevant in determining the highly invasive behaviour of gliomas, though it does not behave as an independent prognostic factor for survival.
胶质瘤的侵袭性涉及肿瘤细胞与富含透明质酸（HA）的脑细胞外基质的附着。CD44是HA的主要受体，以标准分子（CD44s）或变体（CD44v）的形式存在。我们进行了一项回顾性研究，以评估CD44s、异构体CD44v3、v4/5和v6的表达模式，并将它们的表达与临床-解剖病理学参数及生存率相关联。
采用免疫组织化学方法评估了84例胶质瘤中这些分子的表达。
在任何肿瘤中均未检测到CD44v的表达。84例胶质瘤中有70例（83.3%）检测到肿瘤细胞的CD44s染色。在39例胶质母细胞瘤（GBM）中有23例（59.0%）超过70%的细胞被染色，而在21例低级别星形细胞瘤（LGA）中只有2例（9.5%）显示高表达。在多变量分析中考虑预后变量时，CD44与组织学分级之间的关联依然存在。CD44的高表达与较差的总生存率相关；然而，Cox分析表明生存率与CD44无关。
我们的结果表明，CD44s的过表达可能与确定胶质瘤的高度侵袭性行为有关，尽管它并非生存的独立预后因素。
