果蝇嗅觉器官中气味结合蛋白的表达嵌合体

Expression mosaic of odorant-binding proteins in Drosophila olfactory organs.

作者信息

Shanbhag S R, Hekmat-Scafe D, Kim M S, Park S K, Carlson J R, Pikielny C, Smith D P, Steinbrecht R A

机构信息

Max-Planck-Institut für Verhaltensphysiologie, D-82319 Seewiesen, Germany.

出版信息

Microsc Res Tech. 2001 Dec 1;55(5):297-306. doi: 10.1002/jemt.1179.

DOI:10.1002/jemt.1179

PMID:11754509

Abstract

Deciphering the genome of the fruitfly, Drosophila melanogaster, has revealed 39 genes coding for putative odorant-binding proteins (OBPs), more than are known at present for any other insect species. Using specific antibodies, the expression mosaic of five such OBPs (OS-E, OS-F, LUSH, PBPRP2, PBPRP5) on the antenna and maxillary palp has been mapped in the electron microscope. It was found that (1) OBP expression does correlate with morphological sensillum types and subtypes, (2) several OBPs may be co-localized in the same sensillum, and (3) OBP localization is not restricted to olfactory sensilla. The expression of PBPRP2 in antennal epidermis sheds some light on the possible evolution of OBPs.

摘要

对黑腹果蝇基因组的解读发现了39个编码假定气味结合蛋白（OBP）的基因，这比目前已知的任何其他昆虫物种都要多。利用特异性抗体，已在电子显微镜下绘制了5种此类OBP（OS-E、OS-F、LUSH、PBPRP2、PBPRP5）在触角和下颚须上的表达图谱。研究发现：（1）OBP表达确实与形态学上的感器类型和亚型相关；（2）几种OBP可能共定位在同一个感器中；（3）OBP的定位并不局限于嗅觉感器。PBPRP2在触角表皮中的表达为OBP的可能进化提供了一些线索。