Jiao Jipeng, Zhu Rui, Ren Lili, Tao Jing, Luo Youqing
Key Laboratory for Forest Pest Control, College of Forestry, Beijing Forestry University, Beijing, China.
Sino-French Joint Laboratory for Invasive Forest Pests in Eurasia, Beijing Forestry University/French National Research Institute for Agriculture, Food and Environment (INRAE), Beijing, China.
Front Physiol. 2023 Feb 16;14:1123479. doi: 10.3389/fphys.2023.1123479. eCollection 2023.
Insects have highly specialized and sensitive olfactory systems involving several chemosensory genes to locate their mates and hosts or escape from predators. Pine needle gall midge, (Diptera: Cecidomyiidae), has invaded China since 2016 and caused serious damage. Till now, there is no environmentally friendly measure to control this gall midge. Screening molecules with high affinity to target odorant-binding protein to develop highly efficient attractants is a potential pest management method. However, the chemosensory genes in are still unclear. We identified 67 chemosensory-related genes in the transcriptomes of antennae, including 26 OBPs, 2 CSPs, 17 ORs, 3 SNMPs, 6 GRs, and 13 IRs, using high throughput sequencing. Phylogenetic analysis of these six chemosensory gene families among Dipteran was performed to classify and predict the functions. The expression profiles of OBPs, CSPs and ORs were validated by quantitative real-time PCR. 16 of the 26 OBPs were biased expressed in antennae. and were highly expressed in the antenna of unmated male and female adults. The functions of related OBPs and ORs genes were also discussed. These results provide a basis for the functional research on chemosensory genes at the molecular level.
昆虫具有高度专业化且敏感的嗅觉系统，涉及多个化学感应基因，用于定位配偶和宿主或躲避捕食者。松针瘿蚊（双翅目：瘿蚊科）自2016年入侵中国以来造成了严重破坏。到目前为止，尚无环保措施来控制这种瘿蚊。筛选与目标气味结合蛋白具有高亲和力的分子以开发高效引诱剂是一种潜在的害虫管理方法。然而，松针瘿蚊中的化学感应基因仍不清楚。我们使用高通量测序在触角转录组中鉴定了67个化学感应相关基因，包括26个气味结合蛋白（OBPs）、2个化学感受蛋白（CSPs）、17个嗅觉受体（ORs）、3个感觉神经元膜蛋白（SNMPs）、6个味觉受体（GRs）和13个离子型受体（IRs）。对双翅目昆虫中这六个化学感应基因家族进行了系统发育分析，以分类和预测其功能。通过定量实时PCR验证了OBPs、CSPs和ORs的表达谱。26个OBPs中有16个在触角中偏向性表达。[具体基因名称]和[具体基因名称]在未交配的雄性和雌性成虫触角中高表达。还讨论了相关OBPs和ORs基因的功能。这些结果为化学感应基因在分子水平上的功能研究提供了基础。
