Struyf Frank, Posavad Christine M, Keyaerts Els, Van Ranst Marc, Corey Lawrence, Spear Patricia G
Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA.
J Infect Dis. 2002 Jan 1;185(1):36-44. doi: 10.1086/338116. Epub 2001 Dec 14.
Recently, individuals have been identified who possess T cell responses to herpes simplex virus (HSV) antigens despite the absence of detectable anti-HSV antibodies in their serum. The significance of this immune seronegative status is unclear, but it could indicate resistance to overt HSV infection. The aims of the present study were to investigate whether genetic differences in receptors used by HSV for cell entry (herpesvirus entry mediator [HVEM], nectin-1, and nectin-2) could be detected in immune seronegative individuals. Coding polymorphisms were identified in the HVEM and nectin-1 genes. The variant receptor proteins were expressed, and their ability to bind the viral ligand glycoprotein D and to mediate HSV entry after transient transfection into normally resistant cells was compared with that of their wild-type counterparts. HSV entry activity in wild-type and variant forms of the receptors was indistinguishable, which indicates that the polymorphisms observed are unlikely to explain the possible restrictions on HSV replication or spread in immune seronegative individuals.
最近，已鉴定出一些个体，尽管其血清中未检测到抗单纯疱疹病毒（HSV）抗体，但他们对HSV抗原具有T细胞反应。这种免疫血清阴性状态的意义尚不清楚，但可能表明对显性HSV感染具有抵抗力。本研究的目的是调查在免疫血清阴性个体中是否能检测到HSV用于进入细胞的受体（疱疹病毒进入介质[HVEM]、nectin-1和nectin-2）的基因差异。在HVEM和nectin-1基因中鉴定出编码多态性。表达了变异的受体蛋白，并将其与野生型对应物相比，在瞬时转染到正常抗性细胞后结合病毒配体糖蛋白D和介导HSV进入的能力进行了比较。受体野生型和变异型的HSV进入活性没有区别，这表明观察到的多态性不太可能解释免疫血清阴性个体中对HSV复制或传播可能存在的限制。
