Dvoráková D
Vet Med (Praha). 1979 Oct;24(10):603-14.
The possibility of detecting chlamydia by the direct immunofluorescence method was tested in the impression preparation of experimentally infected mice, chick embryos and naturally infected domestic mammals. In comparison with slight-microscope detection, this method was found to be sensitive and expedient. The greatest amount of antigen could be detected in the lungs of mice after intranasal infection already within 6 to 12 hours. In the other organs the findings were less ample; the visualization of the antigen had a good fluorescence brilliance if log 10 KEID50 was more than 3.0. In the tissues of chick embryos, the antigen could be detected in the impression preparations of the allanto-amnionic fluid, embryonal muscle, yolk sac, and chorio-allantoid membrane the fifth day after infection. After the intratracheal infection of ducklings with 1 000 000 infection doses, only individual specifically radiating cells with a low brilliance of fluorescence were detected in the organ preparations of ducklings killed 22 t0 57 days after infection. The method proved to be suitable for the detection of chlamydia in the organs of naturally infected domestic mammals in which a great amount of specifically fluorescing high-brilliance cells were found.
采用直接免疫荧光法检测经实验感染的小鼠、鸡胚以及自然感染的家养哺乳动物印片标本中衣原体的可能性。与显微镜检测相比，该方法灵敏且便捷。鼻内感染后6至12小时内即可在小鼠肺中检测到大量抗原。在其他器官中，发现的抗原较少；若log 10 KEID50大于3.0，则抗原的可视化具有良好的荧光亮度。在鸡胚组织中，感染后第5天可在尿囊 - 羊水、胚胎肌肉、卵黄囊和绒毛尿囊膜的印片标本中检测到抗原。用1000000个感染剂量对雏鸭进行气管内感染后，在感染后22至57天处死的雏鸭器官标本中，仅检测到个别荧光亮度较低的特异性发光细胞。该方法被证明适用于检测自然感染的家养哺乳动物器官中的衣原体，在这些动物器官中发现了大量特异性荧光高亮度细胞。
