Bester K, Bordin G, Rodriguez A, Schimmel H, Pauwels J, Van Vyncht G
European Community-Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Geel, Belgium.
Fresenius J Anal Chem. 2001 Oct;371(4):550-5. doi: 10.1007/s002160101020.
A high-performance liquid chromatographic method, with electrospray ionisation tandem mass spectrometry (HPLC-ESI-MS-MS) for detection, has been developed for the determination of thiabendazole, carbendazime, and phenylurea pesticides in fruit matrices. During the validation process the method was tested for matrix effects, blanks, and the stability of the system. Considerable unspecific matrix effects in the ESI (+) process were detected by comparing standard calibration, and matrix calibration, although blank values were very low and the specific calibration functions showed only small standard deviations. This effect was overcome by using a more complex clean-up, i.e. an additional size-exclusion step.
已开发出一种采用电喷雾电离串联质谱检测的高效液相色谱法（HPLC-ESI-MS-MS），用于测定水果基质中的噻菌灵、多菌灵和苯基脲类农药。在验证过程中，对该方法进行了基质效应、空白和系统稳定性测试。通过比较标准校准和基质校准，发现在电喷雾电离（+）过程中存在相当大的非特异性基质效应，尽管空白值非常低，且特定校准函数的标准偏差很小。通过采用更复杂的净化方法，即增加一个尺寸排阻步骤，克服了这种效应。
