从严重致病性羊蹄腐烂病中分离并鉴定一种新型螺旋体。
Isolation and characterisation of a novel spirochaete from severe virulent ovine foot rot.
作者信息
Demirkan I, Carter S D, Winstanley C, Bruce K D, McNAIR N M, Woodside M, Hart C A
机构信息
Departments of Veterinary Immunology and *Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool, †Division of Life Sciences, Franklin-Wilkins Building, Kings College, London, ‡Veterinary Research Laboratory, Belfast, Northern Ireland and §Clare Veterinary Group, Ballyclare, Northern Ireland.
出版信息
J Med Microbiol. 2001 Dec;50(12):1061-1068. doi: 10.1099/0022-1317-50-12-1061.
A novel spirochaete was isolated from a case of severe virulent ovine foot rot (SVOFR) by immunomagnetic separation with beads coated with polyclonal anti-treponemal antisera and prolonged anaerobic broth culture. The as yet unnamed treponeme differs considerably from the only other spirochaete isolated from ovine foot rot as regards morphology, enzymic profile and 16S rDNA sequence. On the basis of 16S rDNA, it was most closely related to another unnamed spirochaete isolated from cases of bovine digital dermatitis in the USA, raising the possibility of cross-species transmission. Further information is required to establish this novel ovine spirochaete as the cause of SVOFR.
通过用包被多克隆抗密螺旋体抗血清的磁珠进行免疫磁分离和延长厌氧肉汤培养，从一例严重恶性羊足腐病（SVOFR）病例中分离出一种新型螺旋体。就形态、酶谱和16S rDNA序列而言，这种尚未命名的密螺旋体与从羊足腐病中分离出的唯一另一种螺旋体有很大不同。基于16S rDNA，它与从美国牛趾间皮炎病例中分离出的另一种未命名螺旋体关系最为密切，这增加了跨物种传播的可能性。需要更多信息来确定这种新型羊螺旋体是否为SVOFR的病因。
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