Soukup S W, Au W
Humangenetik. 1975 Oct 7;29(4):319-28. doi: 10.1007/BF00394194.
The effect of ENU on (A) human chromosomes from blood lymphocyte cultures in vitro, and on (B) rat and mouse bone marrow chromosomes in vivo, was investigated. Doses of 25, 50, 100 and 200 mug/ml were tested in vitro and cells with chromosome breakage were found to be dose dependent. Chromosome damage was also dependent on time; maximum damage was seen when cells were treated 2--6 hrs before harvest. Two doses of 100 and 200 mg/kg were studied in rat and mouse in vivo and a dose effect could be shown in both species. The highest number of abnormal cells was found 6 hrs after treatment; there was a sharp decrease at 18 hrs and thereafer. Types of aberrations were also analyzed, in both in vitro and in vivo studies.
研究了ENU对（A）体外血液淋巴细胞培养中的人类染色体以及（B）体内大鼠和小鼠骨髓染色体的影响。在体外试验了25、50、100和200微克/毫升的剂量，发现有染色体断裂的细胞呈剂量依赖性。染色体损伤也取决于时间；在收获前2至6小时处理细胞时可见最大损伤。在大鼠和小鼠体内研究了100和200毫克/千克的两个剂量，并且在两个物种中都显示出剂量效应。处理后6小时发现异常细胞数量最多；在18小时及之后急剧下降。在体外和体内研究中还分析了畸变类型。
