Izumi S, Penrose J M, More D G, Nelson D S
Int Arch Allergy Appl Immunol. 1975;49(5):573-84. doi: 10.1159/000231439.
Subcutaneous injection of ovalbumin (OA) into mice immunized with OA in Freund's complete adjuvant was followed by an increase in the numbers of peritoneal macrophages synthesizing DNA, determined by autoradiography. The effect was immunologically specific. The increase was followed by an increase in the numbers of peritoneal macrophages; the numbers of peritoneal lymphocytes also increased. Injection of OA into immunized or normal mice was followed by a blood monocytosis. Increased DNA synthesis, determined by liquid scintillation counting, occurred in spleen or lymph node cells from immunized mice, cultured with OA. Diluted supernatants from such cultures, injected intravenously into normal mice, caused increases in the numbers of DNA-synthesizing peritoneal macrophages. Similarly, supernatants from concanavalin A stimulated spleen cells, freed of Con A, also caused an increase in DNA-synthesizing macrophages.
将卵清蛋白（OA）皮下注射到用弗氏完全佐剂免疫过的小鼠体内后，通过放射自显影法测定发现，合成DNA的腹膜巨噬细胞数量增加。该效应具有免疫特异性。巨噬细胞数量增加之后，腹膜淋巴细胞数量也增加。将OA注射到免疫或正常小鼠体内后会出现血液单核细胞增多。用OA培养免疫小鼠的脾细胞或淋巴结细胞，通过液体闪烁计数法测定发现DNA合成增加。将此类培养物的稀释上清液静脉注射到正常小鼠体内，会使合成DNA的腹膜巨噬细胞数量增加。同样，刀豆球蛋白A刺激过的脾细胞的上清液（去除了刀豆球蛋白A）也会使合成DNA的巨噬细胞数量增加。
