Tamerius J, Hellström I, Hellström K E
Int J Cancer. 1975 Sep 15;16(3):456-64. doi: 10.1002/ijc.2910160313.
Previous work has shown that lymph-node cells from pregnant BALB/c mice can kill syngeneic tumor cells in a microcytotoxicity assay, and that sera from pregnant BALB/c mice can block (inhibit) that effect. The present study shows that passage of such sera through immunoadsorbents charged with anti-mouse IgG2a or IgG2b removed the blocking activity, and that this could be recovered in the eluates; passage through an anti-IgG1 immunoadsorbent was less effective in decreasing blocking serum activity and adsorption to immunoadsorbent charged with anti-IgM or anti-IgA did not remove it at all. Passage of blocking serum through an immunoadsorbent coupled with antibodies from a rabbit anti-mouse embryo antiserum removed all of its blocking activity, and this could be recovered in the eluates. The findings are compatible with the hypothesis that the blocking factors operating in this system are largely complexes between embryonic antigens and IgG2a and IgG2b.
先前的研究表明，在微量细胞毒性试验中，来自怀孕BALB/c小鼠的淋巴结细胞能够杀死同基因肿瘤细胞，并且怀孕BALB/c小鼠的血清能够阻断（抑制）这种效应。本研究表明，此类血清通过装有抗小鼠IgG2a或IgG2b的免疫吸附剂后，阻断活性消失，且这种活性可在洗脱液中恢复；通过抗IgG1免疫吸附剂对降低阻断血清活性的效果较差，而吸附到装有抗IgM或抗IgA的免疫吸附剂上则根本不会消除其阻断活性。阻断血清通过与兔抗小鼠胚胎抗血清抗体偶联的免疫吸附剂后，其所有阻断活性均消失，且这种活性可在洗脱液中恢复。这些发现与以下假设相符，即在该系统中起作用的阻断因子主要是胚胎抗原与IgG2a和IgG2b之间的复合物。
