Lancz G J, Bradstreet J J
Arch Virol. 1976;52(1-2):37-46. doi: 10.1007/BF01317863.
The relationships between the environmental pH and the replication and spread of herps simplex virus (HSV) infection in rabbit skin (RS) cell cultures was studies. Relative to the plaque formation at pH7.0, incubation of RS cells with medium adjusted to pH 6.6 resulted in a 50 to 70 per cent decrement in plaque number. The addition of overlay media adjusted to pH 6.0 or 6.3 precluded HSV plaque formation. Select HSV-1 (type 1) and HSV (type 2) strains readily survived a 3 day incubation with medium adjusted to pH 6.3 as demonstrated by plaque production following a medium shift to pH 7.0. Rs cells incubated with medium at pH 6.3 did not replicate. The survival of RS cells incubated for 3 days with medium adjusted to pH 6.3 was demonstrated by renewed cell proliferation following a medium change from pH 6.3 to 7.0. The progeny virus yields of two HSV strains replicating in RS cells incubated with medium adjusted to pH 6.3 or 7.0 were equivalent at 24 or 48 hours post infection and were indicative of a productive infection. The inhibition of HSV plaque formation at pH 6.3 was not due to an alteration of cell receptors but was the result of an inhibition in the cell to cell spread of the virus. These results are discussed with regard to the possibility that the decline in pH associated with the inflammatory response may serve as a host defense against HSV infections.
研究了环境pH值与单纯疱疹病毒（HSV）在兔皮肤（RS）细胞培养物中的复制和传播之间的关系。相对于pH7.0时的蚀斑形成，用pH值调至6.6的培养基孵育RS细胞，蚀斑数量减少了50%至70%。添加pH值调至6.0或6.3的覆盖培养基可阻止HSV蚀斑形成。选定的HSV-1（1型）和HSV（2型）毒株在pH值调至6.3的培养基中孵育3天后仍能存活，将培养基pH值调至7.0后产生蚀斑即可证明。在pH6.3的培养基中孵育的RS细胞不进行复制。将培养基从pH6.3改为7.0后，细胞重新增殖，证明在pH6.3的培养基中孵育3天的RS细胞能够存活。在pH6.3或7.0的培养基中孵育的RS细胞中复制的两种HSV毒株，其子代病毒产量在感染后24或48小时相当，表明是生产性感染。pH6.3时HSV蚀斑形成的抑制不是由于细胞受体的改变，而是病毒细胞间传播受到抑制的结果。结合炎症反应相关的pH值下降可能作为宿主抵御HSV感染的一种防御机制这一可能性，对这些结果进行了讨论。
