Colberg-Poley A M, Isom H C, Rapp F
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5948-51. doi: 10.1073/pnas.76.11.5948.
The ability of human cytomegalovirus to stimulate replication of herpes simplex virus type 2 (HSV-2) was examined. The system used involved HSV-2-infected human embryonic lung cells under conditions (39.5-40 degrees C) in which HSV-2 remains undetectable. Reactivation of HSV-2 was maximal and persisted for the longest duration when cultures were superinfected with 0.02 plaque-forming unit of human cytomegalovirus per cell. Infectious HSV-2 appeared 2 days after superinfection with human cytomegalovirus and ranged from 10(2) to 10(6) plaque-forming units per culture. Virus reactivated from these cultures was neutralized by rabbit immune serum produced against HSV-2. The specificity of this interaction was demonstrated by various criteria: production of HSV-2 was not observed in cultures treated with mock infecting fluid, and inactivation of human cytomegalovirus by heat, ultraviolet irradiation, or immune serum prior to superinfection eliminated its ability to induce HSV-2 replication. These results sugges that interaction between these two human herpesviruses may be of importance in herpesvirus latency in vivo.
研究了人巨细胞病毒刺激2型单纯疱疹病毒（HSV - 2）复制的能力。所使用的系统涉及在HSV - 2无法检测到的条件（39.5 - 40摄氏度）下感染了HSV - 2的人胚肺细胞。当每细胞用0.02个空斑形成单位的人巨细胞病毒进行超感染时，HSV - 2的重新激活达到最大值且持续时间最长。在用人巨细胞病毒进行超感染后2天出现有感染性的HSV - 2，每个培养物中其范围为10²至10⁶个空斑形成单位。从这些培养物中重新激活的病毒被针对HSV - 2产生的兔免疫血清中和。这种相互作用的特异性通过各种标准得以证明：在用模拟感染液处理的培养物中未观察到HSV - 2的产生，并且在超感染前通过加热、紫外线照射或免疫血清使人巨细胞病毒失活消除了其诱导HSV - 2复制的能力。这些结果表明这两种人类疱疹病毒之间的相互作用可能在体内疱疹病毒潜伏中具有重要意义。
