Ulker H, Kanter M, Gökdal O, De Avila D M, Reeves J J
Yüzüncü Yil University, Ziraat Fakültesi, Van, Turkey.
Dtsch Tierarztl Wochenschr. 2001 Nov;108(11):459, 462-4.
Two recombinant luteinizing hormone releasing hormone (LHRH) fusion proteins were evaluated for their effectiveness in suppression of testicular development and histology by injecting together. Recombinant fusion proteins, ovalbumin-LHRH-7 and thioredoxin-LHRH-7, were generated using recombinant DNA technology and expressed in E. Coli. Eleven ram lambs ranked by age and body weight were randomly assigned to receive either ovalbumin and thioredoxin recombinant protein mixture (control group, n = 5) or ovalbumin-LHRH-7 and thioredoxin-LHRH-7 recombinant fusion protein mixture, anti-LHRH vaccine, (immunization group, n = 6). Animals in each group were weaned at 17 wk of age and were injected (primary immunization) with either mixture at 18 wk of age. Both groups received a booster immunization 8 wks later (26 wk of age). Scrotal circumference, scrotal length, testicular diameter and testicular length were measured in both groups every other week. All animals were slaughtered at 36 wk of age. Immediately after slaughter, a small testicular tissue was taken and processed for histological examination. In the ram lambs in immunization group scrotal circumference and testicular diameter increased steadily until second booster and then remained as a plateau until the end of the experiment. The differences in scrotal circumferences and testicular diameter were significant between the two groups during the last three weeks of the study (p < 0.05). There were no differences in testicular and scrotal length throughout the study (p > 0.05). Seminiferous tubules lost their regular shape and were decreased in diameter in immunization group. Although a few spermatozoa were seen in some tubules, in general, there were atrophy of the seminiferous tubules and loss of spermatogenesis, nevertheless, it seemed that animals in this group were potentially fertile.
通过共同注射两种重组促黄体生成激素释放激素（LHRH）融合蛋白，评估它们在抑制睾丸发育和组织学方面的有效性。使用重组DNA技术制备重组融合蛋白卵清蛋白-LHRH-7和硫氧还蛋白-LHRH-7，并在大肠杆菌中表达。按年龄和体重排序的11只公羊羔被随机分配接受卵清蛋白和硫氧还蛋白重组蛋白混合物（对照组，n = 5）或卵清蛋白-LHRH-7和硫氧还蛋白-LHRH-7重组融合蛋白混合物（抗LHRH疫苗，免疫组，n = 6）。每组动物在17周龄断奶，并在18周龄时注射（初次免疫）上述两种混合物中的一种。两组均在8周后（26周龄）进行加强免疫。每隔一周测量两组的阴囊周长、阴囊长度、睾丸直径和睾丸长度。所有动物在36周龄时屠宰。屠宰后立即取一小块睾丸组织进行组织学检查。在免疫组的公羊羔中，阴囊周长和睾丸直径在第二次加强免疫前稳步增加，然后保持稳定直至实验结束。在研究的最后三周，两组之间的阴囊周长和睾丸直径差异显著（p < 0.05）。在整个研究过程中，睾丸和阴囊长度没有差异（p > 0.05）。免疫组的生精小管失去了正常形状，直径减小。尽管在一些小管中可见少量精子，但总体而言，生精小管萎缩，精子发生丧失，然而，该组动物似乎仍有生育能力。
