从波兰健康犊牛中分离出的产坏死毒素大肠杆菌（NTEC）菌株的特性分析。

Characterization of necrotoxigenic Escherichia coli (NTEC) strains isolated from healthy calves in Poland.

作者信息

Osek J

机构信息

Department of Microbiology, National Veterinary Research Institute, Pulawy, Poland.

出版信息

J Vet Med B Infect Dis Vet Public Health. 2001 Nov;48(9):641-6. doi: 10.1046/j.1439-0450.2001.00481.x.

DOI:10.1046/j.1439-0450.2001.00481.x

PMID:11765799

Abstract

Faecal samples from 132 healthy, 4-8-week-old calves from four different farms were examined for necrotoxigenic Escherichia coli (NTEC) producing the cytotoxic necrotizing factors type 1 (CNF1) and type 2 (CNF2). CNF2 genes were detected by polymerase chain reaction in 24 (6.1%) of the 396 E. coli strains tested; these strains were found in 18 (13.6%) calves used in the study. None of the 396 E. coli isolates examined possessed the gene encoding CNF1. Overall, 28.8% of E. coli examined expressed the F17 fimbrial antigen. A strong association between CNF2 toxin and F17 fimbriae was found (62.5% of CNF2-positive strains were F17-positive). Moreover, six out of 24 NTEC strains had the Stx1 or the Stx2 shiga toxin genes, and three additional isolates possessed the eae genetic marker of the intimin protein.

摘要

对来自四个不同农场的132头4 - 8周龄健康犊牛的粪便样本进行检测，以查找产生细胞毒性坏死因子1型（CNF1）和2型（CNF2）的产坏死毒素大肠杆菌（NTEC）。在396株受试大肠杆菌菌株中，通过聚合酶链反应检测到24株（6.1%）含有CNF2基因；在参与研究的18头（13.6%）犊牛中发现了这些菌株。所检测的396株大肠杆菌分离株均未携带编码CNF1的基因。总体而言，28.8%的受试大肠杆菌表达F17菌毛抗原。发现CNF2毒素与F17菌毛之间存在强关联（62.5%的CNF2阳性菌株为F17阳性）。此外，24株NTEC菌株中有6株含有stx1或stx2志贺毒素基因，另有3株分离株具有内膜蛋白intimin的eae基因标记。