从健康奶牛和牛犊中分离出的产坏死毒素大肠杆菌CNF1+和CNF2+的分布及特性

Distribution and characterization of faecal necrotoxigenic Escherichia coli CNF1+ and CNF2+ isolated from healthy cows and calves.

作者信息

Blanco M, Blanco J E, Mora A, Blanco J

机构信息

Department of Microbiology and Parasitology, School of Veterinary Science, University of Santiago de Compostela, Lugo, Spain.

出版信息

Vet Microbiol. 1998 Jan 16;59(2-3):183-92. doi: 10.1016/s0378-1135(97)00191-0.

DOI:10.1016/s0378-1135(97)00191-0

PMID:9549858

Abstract

Faecal swabs obtained from a random sample of 268 cows and 90 calves on 19 Lugo (northwestern Spain) farms were examined for necrotoxigenic Escherichia coli (NTEC) producing the cytotoxic necrotizing factors type 1 (CNF1) and type 2 (CNF2). We found NTEC CNF1+ and CNF2+ on 11% and 95% of the farms, respectively, NTEC producing CNF2 were significantly more frequently isolated from calves (58%) than from cows (17%) (P < 0.001). The proportion of animals colonized with CNF2+ strains on each farm ranged from 0% to 60%. NTEC strains producing CNF2 isolated from healthy cattle belonged to 27 O serogroups; however, 64% were of one of 12 serogroups (O2, O8, O8-O75, O14, O15, O55, O86, O88, O115, O121, O147, and O168). Furthermore, the serogroups determined in CNF2+ strains isolated from cows (O2, O8, and O14) were different from those found in NTEC producing CNF2 isolated from calves (O8-O75, O15, O55, O86, O88, O115 and O147).

摘要

从西班牙西北部卢戈省19个农场随机抽取的268头奶牛和90头犊牛中采集粪便拭子，检测产细胞毒素坏死因子1型（CNF1）和2型（CNF2）的产坏死毒素大肠杆菌（NTEC）。我们分别在11%和95%的农场中发现了CNF1+和CNF2+的NTEC，产CNF2的NTEC从犊牛中分离出来的频率（58%）显著高于奶牛（17%）（P<0.001）。每个农场中被CNF2+菌株定植的动物比例在0%至60%之间。从健康牛分离出的产CNF2的NTEC菌株属于27个O血清型；然而，64%属于12个血清型之一（O2、O8、O8-O75、O14、O15、O55、O86、O88、O115、O121、O147和O168）。此外，从奶牛分离出的CNF2+菌株中确定的血清型（O2、O8和O14）与从犊牛分离出的产CNF2的NTEC中发现的血清型（O8-O75、O15、O55、O86、O88、O115和O147）不同。