Okuda K, Ito K, Miyake K, Morita M, Ogonuki M
J Clin Microbiol. 1975 Jan;1(1):96-101. doi: 10.1128/jcm.1.1.96-101.1975.
A large-scale procedure for purification and concentration of Japanese encephalitis virus vaccine by a continuous-flow isopycnic banding technique in a sucrose density gradient solution, using a K-III zonal centrifuge rotor, is presented. The quality of zonal-purified vaccine was compared with commercial and Japanese National Institutes of Health reference vaccines for antigenicity, immunodiffusion, and allergic encephalitogenicity tests to show its high purity.
本文介绍了一种使用K-III区带离心机转子，在蔗糖密度梯度溶液中通过连续流等密度区带离心技术大规模纯化和浓缩日本脑炎病毒疫苗的方法。将区带纯化疫苗的质量与市售疫苗及日本国立卫生研究院的参考疫苗进行了抗原性、免疫扩散和过敏性致脑炎试验比较，以证明其高纯度。
