Gupta R K, Misra C N, Gupta V K, Saxena S N
Japanese Encephalitis Vaccine Section, Central Research Institute, Kasauli, India.
Vaccine. 1991 Dec;9(12):865-7. doi: 10.1016/0264-410x(91)90004-p.
The efficiency of production of purified Japanese encephalitis (JE) vaccine from mouse brains was increased by reprocessing the brain material after recovery of the virus and by pooling of a few extra fractions after zonal ultracentrifugation. By the routine production method, one mouse yielded approximately 2.5 doses of the vaccine while the improved method gave about five doses from each mouse. All the batches of purified JE vaccine made by improved technology passed all the quality control tests as specified by the Minimum Requirements for biological products of the Japanese Government and World Health Organization.
通过在病毒回收后对脑材料进行再处理以及在区带超速离心后合并一些额外的级分，提高了从小鼠脑生产纯化日本脑炎（JE）疫苗的效率。按照常规生产方法，一只小鼠可产出约2.5剂疫苗，而改进后的方法每只小鼠可产出约5剂。采用改进技术生产的所有批次纯化JE疫苗均通过了日本政府和世界卫生组织生物制品最低要求规定的所有质量控制测试。
