皮肤癣菌感染豚鼠皮肤鳞屑的定量测定。
Quantitative assay of dermatophyte-infected guinea pig skin scales.
作者信息
Sinski J T, Pokrifchak J S, Kelley L M
出版信息
J Clin Microbiol. 1975 Feb;1(2):150-3. doi: 10.1128/jcm.1.2.150-153.1975.
Abstract
A technique to obtain homogeneous suspensions of fungal particles in scrapings from infected skin was devised that permits delivering uniform inocula to culture media being evaluated for suitability as primary isolation media for dermatophytes. The technique consisted of trypsinization of the scales, utilizing scrapings from lesions experimentally induced with an isolate of Trichophyton mentagrophytes var. granulare on guinea pigs. Optimal conditions for dislodging the fungal particles were assessed by colony counts. One-hour treatments with 0.5% (1:300) trypsin yielded the highest counts.
摘要
设计了一种从受感染皮肤刮屑中获得真菌颗粒均匀悬浮液的技术，该技术可将均匀接种物接种到正在评估其作为皮肤癣菌初代分离培养基适用性的培养基中。该技术包括对鳞屑进行胰蛋白酶处理，使用由须癣毛癣菌颗粒变种分离株在豚鼠身上实验性诱导产生的病变刮屑。通过菌落计数评估去除真菌颗粒的最佳条件。用0.5%（1:300）胰蛋白酶处理1小时产生的菌落数最高。
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本文引用的文献
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