Dichmann S, Herouy Y, Purlis D, Rheinen H, Gebicke-Härter P, Norgauer J
Department of Dermatology, University of Freiburg, Germany.
Inflamm Res. 2001 Nov;50(11):529-33. doi: 10.1007/PL00000230.
Dendritic cells (DCs) are considered as the principle initiators of immune responses by virtue of their ability to migrate into target sites, process antigens and activate naive T cells. Here, the chemotactic activity and intracellular signaling of fractalkine was analyzed and compared to well known chemotaxins.
The mRNA-expression of G protein-coupled CX3CR1 was analyzed by RT-PCR. Chemotaxis was measured in 48-well Boyden chambers and actin polymerization by flow cytometry.
The mRNA-expression of CX3CR1 in immature and mature DCs was revealed. Fractalkine elicited actin polymerization and chemotaxis in a dose-dependent manner in DCs independent of their state of maturation.
These results show that immature and mature DCs express mRNA for the CX3CRI and that fractalkine induces chemotaxis and migration associated actin polymerization in immature as well as in mature DCs, contrasting with the action of other chemokines such as RANTES or MIP-3beta which act only on distinct maturation states of DCs.
树突状细胞（DCs）因其能够迁移至靶位点、处理抗原并激活初始T细胞的能力，被视为免疫反应的主要启动者。在此，分析了趋化因子的趋化活性和细胞内信号传导，并与知名趋化因子进行了比较。
通过逆转录聚合酶链反应（RT-PCR）分析G蛋白偶联CX3CR1的mRNA表达。在48孔博伊登小室中测量趋化性，并通过流式细胞术检测肌动蛋白聚合。
揭示了未成熟和成熟DCs中CX3CR1的mRNA表达。趋化因子在DCs中以剂量依赖性方式引发肌动蛋白聚合和趋化性，且与其成熟状态无关。
这些结果表明，未成熟和成熟DCs均表达CX3CR1的mRNA，并且趋化因子在未成熟和成熟DCs中均诱导趋化性以及与迁移相关的肌动蛋白聚合，这与其他趋化因子如RANTES或MIP-3β的作用形成对比，后者仅作用于DCs的不同成熟状态。
