School of Biological Sciences and Biotechnology, Murdoch University, Western Australia 6150, Australia.
Anim Genet. 2010 Dec;41(6):662-5. doi: 10.1111/j.1365-2052.2010.02044.x.
The camel racing industry would have added value in being able to assign parentage with high certainty. This study was aimed at assessing and applying microsatellite multiplexes to construct a parentage testing system for camels. An efficient system of 17 loci from 700 camel samples was used to construct a database of unrelated adults. Based on this, we estimated measures of polymorphism among the markers. In three multiplex reactions, we detected a total of 224 alleles, with 5–23 alleles/locus (mean = 13.18 ± 6.95 SD) and an average heterozygosity (HE) of 0.54 (range 0.032–0.905). The total parentage exclusion probability was 0.99999 for excluding a candidate parent from parentage of an arbitrary offspring, given only the genotype of the offspring, and 0.9999 for excluding a candidate parent from parentage of an arbitrary offspring, given the genotype of the offspring and the other parent. We used 15 juveniles for parentage testing, as well as 17 sires (bull camels) and 21 dams (cows). In the case of parentage assignment, the microsatellite panel assigned all 15 offspring parentage with high confidence. Overall, these findings offer a set of microsatellite markers that are easy, simple and highly informative for parentage testing in camels.
骆驼竞赛行业将能够非常确定地确定亲子关系，从而增加价值。本研究旨在评估和应用微卫星多重扩增技术，为骆驼构建亲子关系测试系统。我们使用来自 700 个骆驼样本的 17 个高效位点来构建一个无亲缘关系的成年骆驼数据库。在此基础上，我们评估了标记之间的多态性。在三个多重反应中，我们共检测到 224 个等位基因，每个位点有 5-23 个等位基因（平均值为 13.18 ± 6.95 SD），平均杂合度（HE）为 0.54（范围为 0.032-0.905）。仅根据后代的基因型，排除候选亲本作为任意后代亲子关系的总排除概率为 0.99999；仅根据后代和另一个亲本的基因型，排除候选亲本作为任意后代亲子关系的总排除概率为 0.9999。我们使用 15 头幼崽进行亲子关系测试，以及 17 头公牛（种公驼）和 21 头母牛（母驼）。在亲子关系分配方面，微卫星面板非常有信心地确定了所有 15 头后代的亲子关系。总的来说，这些发现为骆驼的亲子关系测试提供了一套简单、简单且信息量丰富的微卫星标记。
