一种使用猪传染性胃肠炎病毒核蛋白重组抗原的血清学诊断酶联免疫吸附测定法。
A serodiagnostic ELISA using recombinant antigen of swine transmissible gastroenteritis virus nucleoprotein.
作者信息
Liu C, Kokuho T, Kubota T, Watanabe S, Inumaru S, Yokomizo Y, Onodera T
机构信息
Department of Immunology, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
出版信息
J Vet Med Sci. 2001 Nov;63(11):1253-6. doi: 10.1292/jvms.63.1253.
A serodiagnostic ELISA utilizing the recombinant nucleoprotein (rN protein) of transmissible gastroenteritis virus (TGEV) was developed, and evaluated by examining a panel of 141 virus neutralization (VN) positive and 101 negative sera. The rN protein-based ELISA (rnELISA) appeared to be highly sensitive and specific (98.6% and 98.0%, respectively) when it was compared to the VN test. The result was similar to that of an ELISA based on purified viral antigens with showing good correlation (R=0.829). No cross-reaction was detected with antisera against porcine epidemic diarrhea virus, hog cholera virus, type A rotavirus, pseudorabies virus and swine vesicular disease virus in this ELISA. The rnELISA can be an alternative for the diagnosis of TGE with a great advantage in antigen preparation.
利用传染性胃肠炎病毒（TGEV）的重组核蛋白（rN蛋白）开发了一种血清诊断ELISA，并通过检测一组141份病毒中和（VN）阳性血清和101份阴性血清进行评估。与VN试验相比，基于rN蛋白的ELISA（rnELISA）似乎具有高度敏感性和特异性（分别为98.6%和98.0%）。结果与基于纯化病毒抗原的ELISA相似，显示出良好的相关性（R=0.829）。该ELISA未检测到与抗猪流行性腹泻病毒、猪霍乱病毒、A型轮状病毒、伪狂犬病病毒和猪水疱病病毒的抗血清发生交叉反应。rnELISA可作为TGE诊断的替代方法，在抗原制备方面具有很大优势。
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