Lara-Romero Rocío, Cerriteño-Sánchez José Luis, Castañeda-Montes María Azucena, Ramírez-Mendoza Humberto, Cuevas-Romero Julieta Sandra
Estancias Posdoctorales por México, Consejo Nacional de Humanidades, Ciencias y Tecnologías, Ciudad de México 03940, Mexico.
Departamento de Microbiología e Inmunología, Facultad de Medicina, Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico.
Pathogens. 2024 Dec 22;13(12):1135. doi: 10.3390/pathogens13121135.
(LPMV) is the etiologic agent of blue eye disease (BED), which affects pigs of all ages, and it has been endemic in central Mexico since the 1980s. To date, no disease control program has been established. Therefore, there is a need for a serological diagnostic method with high sensitivity and specificity. In this study, the recombinant protein NP of LPMV was produced in the BL21 system and then purified using affinity chromatography. The purified protein was used to coat plates for an indirect ELISA assay (iELISA). To determine the sensitivity and specificity of the test, a 2 × 2 contingency table was constructed using positive and negative control sera. The specificity and sensitivity levels were 98.1% and 98.7%, respectively. According to our findings, 45% of serum samples (378/839) were positive, with seropositivity percentages in the analyzed states ranging from 72.5% to 6%. To confirm the presence of antibodies, the indirect immunofluorescence technique was applied to iELISA-positive serum samples. In this study, antibodies against the LPMV nucleoprotein were detected, indicating that the virus or defective particles may be circulating in Mexican pigs and highlighting the risk of LPMV spreading to disease-free areas.
拉古纳猪瘟病毒（LPMV）是蓝眼病（BED）的病原体，可感染各年龄段的猪，自20世纪80年代以来在墨西哥中部呈地方流行。迄今为止，尚未建立疾病控制计划。因此，需要一种具有高灵敏度和特异性的血清学诊断方法。在本研究中，LPMV的重组蛋白NP在BL21系统中表达，然后通过亲和层析进行纯化。纯化后的蛋白用于包被酶标板进行间接ELISA检测（iELISA）。为了确定该检测方法的灵敏度和特异性，使用阳性和阴性对照血清构建2×2列联表。特异性和灵敏度水平分别为98.1%和98.7%。根据我们的研究结果，45%的血清样本（378/839）呈阳性，在所分析的各州中血清阳性率从72.5%到6%不等。为了确认抗体的存在，将间接免疫荧光技术应用于iELISA检测呈阳性的血清样本。在本研究中，检测到了针对LPMV核蛋白的抗体，这表明该病毒或缺陷颗粒可能在墨西哥猪群中传播，凸显了LPMV传播到无病地区的风险。
