用于检测猪血清中传染性胃肠炎病毒抗体的酶联免疫吸附测定

Enzyme-linked immunosorbent assay for detection of transmissible gastroenteritis virus antibody in swine sera.

作者信息

Nelson L D, Kelling C L

出版信息

Am J Vet Res. 1984 Aug;45(8):1654-7.

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for detection and quantification of serum antibodies to transmissible gastroenteritis virus (TGEV) in swine. Sera from pigs inoculated with cell culture-origin TGEV or gut-origin TGEV were tested for anti-TGEV antibody by ELISA and by serum virus-neutralization test (NT). The ELISA detected antibody 3 days (av) sooner than did the NT when sera from pigs inoculated with cell culture-origin TGEV were tested and 1 day sooner than did the NT when sera from pigs inoculated with gut-origin TGEV were tested. The ELISA appeared to be more sensitive than the NT, since ELISA was more responsive to low-level antibody and ELISA titers exceeded NT titers.

摘要

开发了一种酶联免疫吸附测定（ELISA）方法，用于检测和定量猪血清中针对传染性胃肠炎病毒（TGEV）的抗体。通过ELISA和血清病毒中和试验（NT）检测接种细胞培养源TGEV或肠道源TGEV的猪血清中的抗TGEV抗体。当检测接种细胞培养源TGEV的猪血清时，ELISA检测抗体的时间比NT早3天（平均），当检测接种肠道源TGEV的猪血清时，ELISA检测抗体的时间比NT早1天。ELISA似乎比NT更敏感，因为ELISA对低水平抗体反应更灵敏，且ELISA滴度超过NT滴度。

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引用本文的文献

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Evaluation of an enzyme-linked immunosorbent assay for the detection of transmissible gastroenteritis virus antibodies.用于检测传染性胃肠炎病毒抗体的酶联免疫吸附测定法的评估

Vet Microbiol. 1987 Jan;13(1):93-7. doi: 10.1016/0378-1135(87)90103-9.

2

Isolation of peplomer glycoprotein E2 of transmissible gastroenteritis virus and application in enzyme-linked immunosorbent assay.传染性胃肠炎病毒纤突糖蛋白E2的分离及其在酶联免疫吸附测定中的应用。

J Clin Microbiol. 1989 Aug;27(8):1754-8. doi: 10.1128/jcm.27.8.1754-1758.1989.

3

An ELISA for the detection of serum antibodies to both transmissible gastroenteritis virus and porcine respiratory coronavirus.一种用于检测抗传染性胃肠炎病毒和猪呼吸道冠状病毒血清抗体的酶联免疫吸附测定法。

Br Vet J. 1991 Jul-Aug;147(4):370-2. doi: 10.1016/0007-1935(91)90010-K.

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