von Holst C, Unglaub W, Anklam E
European Commission, Joint Research Centre, Food Products and Consumer Goods Unit, Ispra (VA), Italy.
J AOAC Int. 2001 Nov-Dec;84(6):1793-8.
Experiments were conducted in a laboratory autoclave and in a commercial rendering plant to set a limit for the R-value of an enzyme-linked immunosorbent assay (ELISA) to prove that animal meal had been sufficiently heat-treated. The immunoassay method is currently the only technique used to check for appropriate sterilization of animal meal. The results of these experiments were statistically assessed using operation characteristic curves and confirmed that insufficiently heat-treated animal meal has an R-value > 2. Results of the trials also demonstrated that the main parameters of sterilization, temperature, and duration strongy influence the R-value, thus indicating that deviation from the target sterilization conditions is verifiable by the ELISA method.
在实验室高压灭菌器和商业提炼厂进行了实验，以设定酶联免疫吸附测定（ELISA）的R值限制，以证明动物饲料已得到充分热处理。免疫测定法是目前唯一用于检查动物饲料是否经过适当灭菌的技术。使用操作特征曲线对这些实验结果进行了统计评估，并证实热处理不充分的动物饲料的R值>2。试验结果还表明，灭菌的主要参数，即温度和持续时间，对R值有很大影响，因此表明通过ELISA方法可以验证是否偏离目标灭菌条件。
