Ofori Jack A, Hsieh Yun-Hwa P
Department of Nutrition, Food and Exercise Sciences, Florida State University, Tallahassee, Florida 32306-1493, USA.
J Agric Food Chem. 2007 Jul 25;55(15):5919-24. doi: 10.1021/jf070034r. Epub 2007 Jun 20.
The feeding of ruminant proteins to ruminants is prohibited in most countries because the practice is thought to be responsible for the spread of bovine spongiform encephalopathy. However, currently available methods to detect ruminant blood products in rendered feedstuffs are inadequate because they lack species specificity, tissue specificity, and are not based on a thermostable analyte. A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for this study that provides reliable and sensitive (0.05-0.5% v/v) detection of bovine blood materials in animal feed. The new sandwich ELISA employs two previously developed monoclonal antibodies (MAbs), Bb6G12 as the capture antibody and biotinylated MAb Bb3D6 as the detecting antibody, and is bovine-specific and blood-specific. The assay is based on the detection of a 60 kDa thermostable protein in bovine blood and provides a useful regulatory tool for monitoring fraudulent labeling or contamination of bovine blood in both heat-processed feedstuffs and unprocessed raw materials.
Bovine; blood; monoclonal antibody; sandwich ELISA.
在大多数国家，反刍动物蛋白质被禁止喂给反刍动物，因为人们认为这种做法是导致牛海绵状脑病传播的原因。然而，目前用于检测制成饲料中的反刍动物血液制品的方法并不充分，因为它们缺乏物种特异性、组织特异性，且并非基于热稳定分析物。本研究开发了一种夹心酶联免疫吸附测定法（ELISA），可对动物饲料中的牛血液材料进行可靠且灵敏（0.05 - 0.5% v/v）的检测。这种新的夹心ELISA使用了两种先前开发的单克隆抗体（MAb），Bb6G12作为捕获抗体，生物素化的MAb Bb3D6作为检测抗体，具有牛特异性和血液特异性。该测定法基于对牛血液中一种60 kDa热稳定蛋白的检测，为监测热处理饲料和未加工原料中牛血液的欺诈性标签或污染提供了一种有用的监管工具。
牛；血液；单克隆抗体；夹心ELISA。
