Kirchner H, Holden H T
J Immunol. 1975 Nov;115(5):1212-6.
Spleen cells from C57BL/6N mice injected with killed Corynebacterium parvum (CP) had a marked growth inhibitory effect on the in vitro proliferation of RBL-5 murine lymphoma cells. It was most marked 12 to 14 days after injection and was usually no longer detectable later than 21 days. It could be demonstrated at effector cell to target ratios between 20:1 and 5:1 at which normal spleen cells had a growth-promoting effect. Addition of CP to an in vitro mixture of spleen cells and tumor cells augmented the inhibitory effect of spleen cells from CP-injected mice although it conferred no inhibitory potential on normal spleen cells. Growth inhibiton by CP spleen cells was not mediated by T cells and various depletion experiments suggested that the effector cells of the phenomenon were macrophages. Spleen cells of CP-injected mice also showed strongly depressed responses to the T cell mitogens PHA and Con A and suppressed the mitogen responses of syngeneic normal spleen cells. The characteristics of the suppressor cells mediating this effect appeared to be very similar to those inhibiting lymphoma cell growth. The responses to LPS were also strongly suppressed in mice injected with 2.1 mg of CP. However, after injection of one-tenth of the dose a relative sparing of the LPS response was noted, whereas the PHA response was still suppressed.
给C57BL/6N小鼠注射灭活的细小棒状杆菌（CP）后，其脾细胞对RBL - 5小鼠淋巴瘤细胞的体外增殖具有显著的生长抑制作用。这种作用在注射后12至14天最为明显，通常在21天之后就不再能检测到。在效应细胞与靶细胞比例为20:1至5:1时可以观察到这种抑制作用，而在此比例下正常脾细胞具有促生长作用。在脾细胞与肿瘤细胞的体外混合物中添加CP可增强注射CP小鼠脾细胞的抑制作用，尽管它对正常脾细胞没有抑制作用。CP脾细胞的生长抑制作用不是由T细胞介导的，各种去除实验表明该现象的效应细胞是巨噬细胞。注射CP小鼠的脾细胞对T细胞有丝分裂原PHA和Con A的反应也明显降低，并抑制了同基因正常脾细胞的有丝分裂原反应。介导这种效应的抑制细胞的特征似乎与抑制淋巴瘤细胞生长的细胞非常相似。注射2.1毫克CP的小鼠对LPS的反应也受到强烈抑制。然而，注射十分之一剂量后，发现LPS反应相对保留，而PHA反应仍然受到抑制。
