利用RAPD标记揭示的 Bambara 花生（Vigna subterranea L.）种质的遗传多样性。
Genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm revealed by RAPD markers.
作者信息
Amadou H I, Bebeli P J, Kaltsikes P J
机构信息
Department of Plant Breeding and Biometry, Agricultural University of Athens, Greece.
出版信息
Genome. 2001 Dec;44(6):995-9. doi: 10.1139/g01-096.
Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm using 25 African accessions from the collection in the International Institute for Tropical Agriculture, Ibadan, Nigeria. Fifty random decamer primers were screened to assess their ability to detect polymorphism in bambara; 17 of them were selected for this study. Considerable genetic diversity was found among the V. subterranea accessions studied. The relationships among the 25 accessions were studied by cluster analysis. The dendrograms showed two main groups of accessions mainly along the lines of their geographic origin. It is concluded that RAPD can be used for germplasm classification in bambara groundnut and hence for improving this crop.
利用随机扩增多态性DNA（RAPD）标记，对来自尼日利亚伊巴丹国际热带农业研究所收集的25份非洲种质资源的 bambara 花生（Vigna subterranea L.）进行遗传多样性评估。筛选了50个随机十聚体引物，以评估它们检测 bambara 花生多态性的能力；其中17个被选用于本研究。在所研究的 V. subterranea 种质资源中发现了相当大的遗传多样性。通过聚类分析研究了这25份种质资源之间的关系。聚类图显示，这些种质资源主要根据其地理来源分为两个主要类群。得出的结论是，RAPD可用于 bambara 花生的种质分类，从而用于改良这种作物。
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