Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom 73140, Thailand.
Genome. 2011 Nov;54(11):898-910. doi: 10.1139/g11-056. Epub 2011 Oct 21.
Bambara groundnut ( Vigna subterranea (L.) Verdc.) is an important African legume crop. In this study, a collection consisting of 240 accessions was analyzed using 22 simple sequence repeat (SSR) markers. In total, 166 alleles were detected, with a mean of 7.59 alleles per locus. Allelic and gene diversities were higher in the west African and Cameroon/Nigeria regions with 6.68 and 6.18 alleles per locus, and 0.601 and 0.571, respectively. The genetic distance showed high similarity between west African and Cameroon/Nigeria accessions. Principal coordinate analyses and neighbor-joining analysis consistently revealed that the majority of west African accessions were grouped with Cameroon/Nigeria accessions, but they were differentiated from east African, central African, and southeast Asian accessions. Population structure analysis showed that two subpopulations existed, and most of the east African accessions were restricted to one subpopulation with some Cameroon/Nigeria accessions, whereas most of the west African accessions were associated with most of the Cameroon/Nigeria accessions in the other subpopulation. Comparison with SSR analysis of other Vigna cultigens, i.e., cultivated azuki bean ( Vigna angularis ) and mungbean ( Vigna radiata ), reveals that the mean gene diversity of Bambara groundnut was lower than azuki bean but higher than mungbean.
斑巴拉 groundnut（Vigna subterranea（L.）Verdc.）是一种重要的非洲豆科作物。本研究利用 22 个简单序列重复（SSR）标记对包含 240 个品系的样本进行了分析。共检测到 166 个等位基因，每个位点的平均等位基因数为 7.59。西非和喀麦隆/尼日利亚地区的等位基因和基因多样性较高，每个位点的等位基因数分别为 6.68 和 6.18，基因多样性分别为 0.601 和 0.571。遗传距离显示，西非和喀麦隆/尼日利亚的品系之间具有高度相似性。主坐标分析和邻接聚类分析均表明，大多数西非品系与喀麦隆/尼日利亚品系聚在一起，但与东非、中非和东南亚的品系存在分化。种群结构分析显示存在两个亚群，大多数东非品系局限于一个亚群，其中一些喀麦隆/尼日利亚品系也存在于该亚群，而大多数西非品系与其他亚群中的大多数喀麦隆/尼日利亚品系有关。与其他 Vigna 栽培种（如栽培的赤豆和绿豆）的 SSR 分析比较表明，斑巴拉 groundnut 的平均基因多样性低于赤豆，但高于绿豆。
