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A simple, rapid method for measurement of acetate in tissue and serum.

作者信息

Richards R G, Mendenhall C L, MacGee J

出版信息

J Lipid Res. 1975 Sep;16(5):395-7.

PMID:1176835
Abstract

A simple and rapid method is described for determining the free acetate concentration in liver and serum. After extraction, the acetate is converted to its benzyl ester by thermal degradation of its benzyldimethylphenylammonium salt in the vaporizer of a gas chromatography. Good quantitation is achieved in the range of 0.033-2.5 mumoles of acetate per gram of liver or per milliliter of serum.

摘要

相似文献

1
A simple, rapid method for measurement of acetate in tissue and serum.
J Lipid Res. 1975 Sep;16(5):395-7.
2
Measurement of acetate in aqueous solutions and plasma by gas phase chromatography using a porous polymer stationary phase.使用多孔聚合物固定相通过气相色谱法测量水溶液和血浆中的乙酸盐。
Ann Clin Biochem. 1978 Jul;15(4):228-32. doi: 10.1177/000456327801500149.
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[Adaptation of a technic of rapid titration of acetate in biological fluids by gas chromatography (author's transl)].[气相色谱法快速滴定生物流体中乙酸盐技术的改编（作者译）]
Ann Biol Clin (Paris). 1980;38(4):227-30.
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Determination of acetate in biological material by vacuum microdistillation and gas chromatography.
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A gas chromatographic method for determination of acetate levels in body fluids.一种用于测定体液中乙酸盐水平的气相色谱法。
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Determination of acetate by gas-liquid chromatography.通过气液色谱法测定乙酸盐。
Methods Enzymol. 1975;35:307-11. doi: 10.1016/0076-6879(75)35169-0.
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[Separation and determination, using a gas-chromatographic method, of vinyl acetate, methyl acetate and methyl alcohol in industrial environments].
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The examination of some vinyl acetate/olefin copolymers by pyrolysis gas chromatography mass spectrometry.通过热解气相色谱 - 质谱联用技术对一些醋酸乙烯酯/烯烃共聚物进行的检测。
J Chromatogr Sci. 1975 Aug 10;13(8):383-5. doi: 10.1093/chromsci/13.8.383.
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Assay of the concentration and 13C enrichment of acetate and acetyl-CoA by gas chromatography-mass spectrometry.采用气相色谱-质谱联用技术测定乙酸盐和乙酰辅酶A的浓度及13C丰度。
Anal Biochem. 1994 Apr;218(1):143-8. doi: 10.1006/abio.1994.1153.
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Determination of sodium monofluoroacetate (compound 1080) in tissues and baits as its benzyl ester by reaction-capillary gas chromatography.
J Anal Toxicol. 1987 Jul-Aug;11(4):140-3. doi: 10.1093/jat/11.4.140.

引用本文的文献

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Measurement of cations, anions, and acetate in serum, urine, cerebrospinal fluid, and tissue by ion chromatography.采用离子色谱法测定血清、尿液、脑脊液和组织中的阳离子、阴离子及乙酸盐。
Physiol Rep. 2018 Apr;6(7):e13666. doi: 10.14814/phy2.13666.
2
Altered proline uptake by mouse liver cells after chronic exposure to ethanol and its metabolites.长期暴露于乙醇及其代谢产物后小鼠肝细胞脯氨酸摄取的改变。
Gut. 1984 Feb;25(2):138-44. doi: 10.1136/gut.25.2.138.
3
Compartmentation of acetyl CoA studied by analysis of tricarboxylic acid cycle acids and 3-hydroxybutyrate in bile of rats given [2,2,2-2H3]ethanol.
通过分析给予[2,2,2-2H3]乙醇的大鼠胆汁中的三羧酸循环酸和3-羟基丁酸来研究乙酰辅酶A的区室化。
Biochem J. 1990 Jan 15;265(2):569-74. doi: 10.1042/bj2650569.