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[气相色谱法快速滴定生物流体中乙酸盐技术的改编(作者译)]

[Adaptation of a technic of rapid titration of acetate in biological fluids by gas chromatography (author's transl)].

作者信息

Brault D, Ragot J

出版信息

Ann Biol Clin (Paris). 1980;38(4):227-30.

PMID:7446876
Abstract

The proposed technic is a rapid method of measurement of acetatemia by simple deproteinisation of plasma, then analysis by gas chromatography. The plasma is routinely saturated with acetate in formic acid solution in order to permit greater sensitivity of reading of usual or subnormal concentrations. The chromatography column is filled with chromosorb WAW impregnated with 15 per cent didecyl phtalate and 2 per cent phosphoric acid. The stationary phase of intermediate polarity permitting satisfactory stability of the chromatograms.

摘要

所提出的技术是一种通过简单的血浆去蛋白化,然后进行气相色谱分析来快速测量乙酸血症的方法。为了提高对正常或低于正常浓度读数的灵敏度,血浆通常在甲酸溶液中用乙酸饱和。色谱柱填充有浸渍了15%邻苯二甲酸二癸酯和2%磷酸的Chromosorb WAW。中间极性的固定相可使色谱图具有令人满意的稳定性。

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