大型质粒的高效精准定点诱变 - Suppr

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超能文献

Efficient and accurate site-directed mutagenesis of large plasmids.

作者信息

Nadin-Davis Susan A

机构信息

Animal Diseases Research Institute, Canadian Food Inspection Agency, Nepean, Ontario, Canada.

出版信息

Methods Mol Biol. 2002;182:45-54. doi: 10.1385/1-59259-194-9:045.

DOI:10.1385/1-59259-194-9:045

PMID:11768974

Abstract

摘要

相似文献

Efficient and accurate site-directed mutagenesis of large plasmids.

Methods Mol Biol. 2002;182:45-54. doi: 10.1385/1-59259-194-9:045.

Rapid PCR site-directed mutagenesis.

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相似文献

Efficient and accurate site-directed mutagenesis of large plasmids.

Methods Mol Biol. 2002;182:45-54. doi: 10.1385/1-59259-194-9:045.

Rapid PCR site-directed mutagenesis.

PCR Methods Appl. 1994 Dec;4(3):S131-6. doi: 10.1101/gr.4.3.s131.

Recombination and site-directed mutagenesis using recombination PCR.

Methods Mol Biol. 1997;67:131-40. doi: 10.1385/0-89603-483-6:131.

Site-directed mutagenesis.

Methods Enzymol. 1998;293:53-71. doi: 10.1016/s0076-6879(98)93007-5.

Cloning of complementary DNA inserts from phage DNA directly into plasmid vector.

Methods Enzymol. 1992;216:508-16. doi: 10.1016/0076-6879(92)16046-m.

Cloning unmodified PCR products using engineered XcmI restriction sites in a portable cassette.

Methods Mol Biol. 1997;67:89-100. doi: 10.1385/0-89603-483-6:89.

Efficient complementary DNA amplification and expression using polymerase chain reaction technology.

Methods Enzymol. 1993;217:102-22. doi: 10.1016/0076-6879(93)17058-d.

Introducing restriction sites into double-stranded plasmid DNA.

Methods Enzymol. 1993;217:295-301. doi: 10.1016/0076-6879(93)17070-l.

A high-yield method for site-directed mutagenesis using polymerase chain reaction and three primers.

Anal Biochem. 1994 May 15;219(1):155-7. doi: 10.1006/abio.1994.1246.

Site-directed mutagenesis by polymerase chain reaction.

Methods Mol Biol. 2002;182:85-94. doi: 10.1385/1-59259-194-9:085.