Chmilevskiĭ D A, Kameneva T O
Biological Research Institute, St. Petersburg State University, Institute of Cytology, RAS, St. Petersburg.
Tsitologiia. 2001;43(10):926-33.
Using methods of light and electron microscopy and of autoradiography, the morphology of cytoplasm in previtellogenic oocytes of tilapia mossambique was studied. Similar to other bony fishes, mitochondria at the early previtellogenic oocytes are mostly located in the perinuclear cytoplasm to be later distributed over the whole volume of growing oocytes. The Golgi complex is poorly developed. In the peripheral regions of the late previtellogenic oocytes, stickform mitochondria, pinocytotic vesicles and microvilli are observed, along with the perioocyte space formation. In the cytoplasm of previtellogenic oocytes polyribosomes appear. No differences in 3H-leucine incorporation intensity was noticed in oocytes of different previtellogenic stages. The characteristic feature of tilapia mossambique previtellogenic oocytes, in comparison with other bony fishes, is the presence of fat droplets in their cytoplasm.
运用光学显微镜、电子显微镜及放射自显影术等方法,对莫桑比克罗非鱼卵黄发生前卵母细胞的细胞质形态进行了研究。与其他硬骨鱼类相似,卵黄发生前早期卵母细胞中的线粒体大多位于核周细胞质中,随后分布于生长中卵母细胞的整个体积内。高尔基体发育不良。在卵黄发生前晚期卵母细胞的周边区域,可观察到棒状线粒体、胞饮小泡和微绒毛,同时形成了卵母细胞周隙。卵黄发生前卵母细胞的细胞质中出现了多核糖体。在不同卵黄发生前期阶段的卵母细胞中,未观察到3H-亮氨酸掺入强度的差异。与其他硬骨鱼类相比,莫桑比克罗非鱼卵黄发生前卵母细胞的特征是其细胞质中存在脂滴。