Chmilevskiĭ D A
Tsitologiia. 2009;51(4):322-8.
Using 3H-uridine dynamics of RNA synthesis in oocytes of tilapia which grow at different temperatures was studied. Intact fishes were cultivated at the temperature 25-26 degrees C, experimental ones -- 19-21 degrees C. Before 3H-uridine injection, experimental fishes were cultivated at low temperature for 5 days, then both intact and experimental fishes were injected by 3H-uridine and experiment was continued for 5 days. Under normal temperature in 8 h after 3H-uridine injection, chromosomes of early previtellogenic oocytes were strongly labeled and RNA migrated to the cytoplasm very fast. Nucleoli of early vitellogenic oocytes were intensively labeled as well. In 1, 2 and 5 days after injection, concentration of labeled RNA in cytoplasm of the oocytes at all stages was increased. Oocytes of the experimental fishes showed low level of RNA synthesis. Probably, this connected with slow migration of 3H-uridine into gonads of fishes, low level RNA synthesis on chromosomes and nucleoli of oocytes and more low migration of labeled RNA from nucleus to cytoplasm of the oocytes.
利用3H-尿苷研究了在不同温度下生长的罗非鱼卵母细胞中RNA合成的动态变化。将完整的鱼在25-26摄氏度的温度下培养，实验鱼在19-21摄氏度下培养。在注射3H-尿苷之前，将实验鱼在低温下培养5天，然后对完整鱼和实验鱼都注射3H-尿苷，并继续实验5天。在正常温度下，注射3H-尿苷后8小时，早期卵黄发生前卵母细胞的染色体被强烈标记，RNA非常迅速地迁移到细胞质中。早期卵黄发生卵母细胞的核仁也被强烈标记。在注射后的第1、2和5天，各阶段卵母细胞细胞质中标记RNA的浓度都有所增加。实验鱼的卵母细胞显示出较低水平的RNA合成。这可能与3H-尿苷向鱼性腺的缓慢迁移、卵母细胞染色体和核仁上较低水平的RNA合成以及标记RNA从细胞核向卵母细胞细胞质的更低迁移率有关。
