[白细胞介素-1通过上调兔下颌髁突软骨细胞诱导型一氧化氮合酶促进体外一氧化氮合成]
[Interleukin-1 increase nitric oxide synthesis through up-regulation of inducible nitric-oxide synthase by rabbit mandibular condylar cartilage cells in vitro].
作者信息
Jiao Y, Ma X, Zhang Z
机构信息
Diagnostic and Treatment Center for Temporomandibular Disorders, Peking University School of Stomatology, Beijing 100081, China.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 2001 Sep;36(5):345-7.
OBJECTIVE
To investigate the influence of IL-1 on the biosynthesis of nitric oxide by rabbit mandibular condylar cartilage (MCC) cells.
METHODS
MCC cells of white rabbits were harvested enzymatically and cultured in DMEM supplemented with 0.4% newborn calf serum. In the experiment, a variety of concentrations of IL-1 were added to the medium. NO production was determined as nitrite accumulation in the medium. The effects of IL-1 on the mRNA levels of inducible nitric-oxide synthase (iNOS) were assessed by mRNA in situ hybridization method.
RESULTS
IL-1 can significantly increase nitric oxide synthesis by MCC cells in a concentration-dependent manner. In addition, normal MCC cells had light expression of iNOS expression and IL-1 (10-100 micrograms/L) promoted the expression of iNOS.
CONCLUSIONS
IL-1 can inhibit the chondrocytes proliferation and induce apoptosis that might be through the way of up-regulation of iNOS expression.
目的
探讨白细胞介素-1（IL-1）对兔下颌髁突软骨（MCC）细胞一氧化氮生物合成的影响。
方法
采用酶解法获取白兔的MCC细胞，并在补充有0.4%新生牛血清的DMEM中培养。实验中，向培养基中添加多种浓度的IL-1。通过测定培养基中亚硝酸盐的积累来确定一氧化氮的产生量。采用mRNA原位杂交法评估IL-1对诱导型一氧化氮合酶（iNOS）mRNA水平的影响。
结果
IL-1能以浓度依赖性方式显著增加MCC细胞的一氧化氮合成。此外，正常MCC细胞中iNOS表达较轻，而IL-1（10 - 100微克/升）可促进iNOS的表达。
结论
IL-1可能通过上调iNOS表达的方式抑制软骨细胞增殖并诱导其凋亡。
Zotero 插件