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与在不同条件下储存的金头鲷腐败相关的假单胞菌属的特性研究。

Characterization of Pseudomonas spp. associated with spoilage of gilt-head sea bream stored under various conditions.

作者信息

Tryfinopoulou P, Tsakalidou E, Nychas G-J E

机构信息

Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Technology, Agricultural University of Athens, Athens 118 55, Greece.

出版信息

Appl Environ Microbiol. 2002 Jan;68(1):65-72. doi: 10.1128/AEM.68.1.65-72.2002.

Abstract

The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish (Sparus aurata) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 Pseudomonas strains isolated from S. aurata stored under different temperatures (at 0, 10, and 20 degrees C) and packaging conditions (air and a modified atmosphere of 40% CO(2)-30% N(2)-30% O(2)). Pseudomonas lundensis was the predominant species, followed by Pseudomonas fluorescens, while Pseudomonas fragi and Pseudomonas putida were detected less frequently. Fluorescent Pseudomonas strains dominated under air conditions, while proteolytic and less lipolytic strains dominated under modified-atmosphere packaging. Different storage conditions appear to govern the selection of pseudomonads in gilt-head sea bream fish.

摘要

研究了在不同条件下储存的金头鲷(Sparus aurata)中假单胞菌的种群动态。对从不同温度(0、10和20摄氏度)和包装条件(空气以及40% CO₂-30% N₂-30% O₂的改良气氛)下储存的金头鲷中分离出的总共106株假单胞菌菌株进行了表型分析和全细胞蛋白质的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳。伦德假单胞菌是优势种,其次是荧光假单胞菌,而弗氏假单胞菌和恶臭假单胞菌的检出频率较低。在空气条件下,荧光假单胞菌菌株占主导,而在改良气氛包装下,蛋白水解性和脂解性较低的菌株占主导。不同的储存条件似乎决定了金头鲷鱼中假单胞菌的选择。

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