Swarup K, Das S, Das V K
Ann Endocrinol (Paris). 1979 Jul-Aug;40(4):403-12.
Rhesus monkey (Macaca mulatta) were subjected to hypercalcaemia by daily intramuscular injections of vitamin D2 (100,000 IU) and by providing them gram soaked in 1% CaCl2 solution for eating and 1% CaCl2 solution (prepared in tap water) for drinking. After 10, 15, 20 and 30 days of such treatment the serum calcium level recorded a rise (18.24 +/- 0.56, 26.20 +/- 1.30, 17.25 +/- 0.25 and 20.50 +/- 0.55 mg/dl respectively) as compared to those of control animals (12.80 +/- 1.00, 12.30 +/- 0.50, 12.70 +/- 0.20 and 12.30 +/- 0.30 mg/dl). Serial sections of thyroid parathyroid complex and isthmus were subjected to selective staining for lcalising the C cells. The structure and behaviour of these cells both under normal and experimental conditions has been studied. Hypercalcaemia resulted in the increase of these cells. Mitotic figures of the C cells were also encountered after 10 days of hypercalcaemia. The specimens subjected to 30 days treatment showed complete degranulation of these cells. Chronic hypercalcaemia inhibits the activity of parathyroid cells which display degenerative changes. The anterior and posterior poles, the peripheral regions of thyroid and isthmus are completely devoid of calcitonin cells.
通过每日肌肉注射维生素D2（100,000国际单位），并给恒河猴（猕猴）提供浸泡在1%氯化钙溶液中的食物以及饮用1%氯化钙溶液（用自来水配制），使其处于高钙血症状态。在进行这种处理10、15、20和30天后，与对照动物（分别为12.80±1.00、12.30±0.50、12.70±0.20和12.30±0.30毫克/分升）相比，血清钙水平有所升高（分别为18.24±0.56、26.20±1.30、17.25±0.25和20.50±0.55毫克/分升）。对甲状腺甲状旁腺复合体和峡部的连续切片进行选择性染色以定位C细胞。研究了这些细胞在正常和实验条件下的结构和行为。高钙血症导致这些细胞数量增加。高钙血症10天后还观察到C细胞的有丝分裂象。经过30天处理的标本显示这些细胞完全脱颗粒。慢性高钙血症抑制甲状旁腺细胞的活性，这些细胞呈现退行性变化。甲状腺和峡部的前后极以及周边区域完全没有降钙素细胞。
