[人舌癌原位移植裸鼠模型的建立及其主要生物学特性研究]
[Human tongue carcinoma model established in nude mice by orthotopic transplantation and studies on its major biological properties].
作者信息
Yang H, Li J, Hu C
机构信息
Hospital of Stomatology, Hubei Medical University, Wuhan 430070.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 1998 Mar;33(2):73-5.
OBJECTIVE
To establish clinically relevant animal model of human tongue carcinoma.
METHODS
Cell suspensions of human tongue squamous cell carcinoma cell line (Tca8113) were inoculated orthotopically into nude mice and the growing tumors were serially passaged by orthotopic transplantation. The oral cavity, mandible and neck of the nude mice were sectioned and examined histologically. The ultrastructure and chromosome of the tumors were also investigated.
RESULTS
- The success rate of transplant was 90%. 2. The mouse-growing tumors exhibited the same identical properties as the donor tumor. 3. Human tongue cancer cells could grow in the homologous oral cavity site of nude mice, extended and metastasized to adjacent tissues and lymph node of the neck.
CONCLUSION
The model can be useful for evaluating biological behavior and new therapeutics for tongue cancer.
目的
建立具有临床相关性的人舌癌动物模型。
方法
将人舌鳞状细胞癌细胞系（Tca8113）的细胞悬液原位接种到裸鼠体内，通过原位移植对生长的肿瘤进行连续传代。对裸鼠的口腔、下颌骨和颈部进行切片并进行组织学检查。还对肿瘤的超微结构和染色体进行了研究。
结果
- 移植成功率为90%。2. 小鼠体内生长的肿瘤表现出与供体肿瘤相同的特性。3. 人舌癌细胞可在裸鼠的同源口腔部位生长，向颈部相邻组织和淋巴结扩展并转移。
结论
该模型可用于评估舌癌的生物学行为和新的治疗方法。
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